2cjq
From Proteopedia
(New page: 200px<br /><applet load="2cjq" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cjq, resolution 2.6Å" /> '''BOVINE VIRAL DIARRHEA...) |
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| - | [[Image:2cjq.gif|left|200px]]<br /><applet load="2cjq" size=" | + | [[Image:2cjq.gif|left|200px]]<br /><applet load="2cjq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cjq, resolution 2.6Å" /> | caption="2cjq, resolution 2.6Å" /> | ||
'''BOVINE VIRAL DIARRHEA VIRUS CP7-R12 RNA-DEPENDENT RNA POLYMERASE'''<br /> | '''BOVINE VIRAL DIARRHEA VIRUS CP7-R12 RNA-DEPENDENT RNA POLYMERASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Viral RNA-dependent RNA polymerases (RdRp) differ from DNA-dependent RNA | + | Viral RNA-dependent RNA polymerases (RdRp) differ from DNA-dependent RNA polymerases, DNA-dependent DNA polymerases, and reverse transcriptases in that RdRps contain "fingertips" consisting of several polypeptide strands in the fingers domain interacting with the thumb domain. The crystal structure of bovine viral diarrhea virus (BVDV) RdRp containing an Asn438 duplication shows that the "N-terminal domain," which occurs only in pestiviruses such as BVDV, interacts with the polymerase component of the same polypeptide chain. This contrasts with the domain swapping observed in the previously determined structure of the BVDV NADL strain RdRp. By comparison with the NADL structure and through the use of biochemical data, it is possible that the N-terminal domain, in conjunction with the fingertips, is required to bind and assist the translocation of the RNA template. The partial disorder of the loop containing the additional Asn438 residue may explain the low replication rate of the recombinant compared with the wild-type virus. |
==About this Structure== | ==About this Structure== | ||
| - | 2CJQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_viral_diarrhea_virus_1 Bovine viral diarrhea virus 1]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http:// | + | 2CJQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_viral_diarrhea_virus_1 Bovine viral diarrhea virus 1]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Becher, P.]] | [[Category: Becher, P.]] | ||
| - | [[Category: Choi, K | + | [[Category: Choi, K H.]] |
[[Category: Gallei, A.]] | [[Category: Gallei, A.]] | ||
| - | [[Category: Rossmann, M | + | [[Category: Rossmann, M G.]] |
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: bovine viral diarrhea virus]] | [[Category: bovine viral diarrhea virus]] | ||
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[[Category: viral polymerase]] | [[Category: viral polymerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:23 2008'' |
Revision as of 14:49, 21 February 2008
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BOVINE VIRAL DIARRHEA VIRUS CP7-R12 RNA-DEPENDENT RNA POLYMERASE
Overview
Viral RNA-dependent RNA polymerases (RdRp) differ from DNA-dependent RNA polymerases, DNA-dependent DNA polymerases, and reverse transcriptases in that RdRps contain "fingertips" consisting of several polypeptide strands in the fingers domain interacting with the thumb domain. The crystal structure of bovine viral diarrhea virus (BVDV) RdRp containing an Asn438 duplication shows that the "N-terminal domain," which occurs only in pestiviruses such as BVDV, interacts with the polymerase component of the same polypeptide chain. This contrasts with the domain swapping observed in the previously determined structure of the BVDV NADL strain RdRp. By comparison with the NADL structure and through the use of biochemical data, it is possible that the N-terminal domain, in conjunction with the fingertips, is required to bind and assist the translocation of the RNA template. The partial disorder of the loop containing the additional Asn438 residue may explain the low replication rate of the recombinant compared with the wild-type virus.
About this Structure
2CJQ is a Single protein structure of sequence from Bovine viral diarrhea virus 1. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.
Reference
The structure of bovine viral diarrhea virus RNA-dependent RNA polymerase and its amino-terminal domain., Choi KH, Gallei A, Becher P, Rossmann MG, Structure. 2006 Jul;14(7):1107-13. PMID:16843892
Page seeded by OCA on Thu Feb 21 16:49:23 2008
Categories: Bovine viral diarrhea virus 1 | RNA-directed RNA polymerase | Single protein | Becher, P. | Choi, K H. | Gallei, A. | Rossmann, M G. | Atp-binding | Bovine viral diarrhea virus | Bvdv | Glycoprotein | Helicase | Hydrolase | Membrane | Nucleotide-binding | Nucleotidyltransferase | Polyprotein | Protease | Rna-dependent rna polymerase | Rna-directed rna polymerase | Serine protease | Thiol protease | Transferase | Transmembrane | Viral polymerase
