2ckz
From Proteopedia
(New page: 200px<br /><applet load="2ckz" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ckz, resolution 3.2Å" /> '''X-RAY STRUCTURE OF RN...) |
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| - | [[Image:2ckz.gif|left|200px]]<br /><applet load="2ckz" size=" | + | [[Image:2ckz.gif|left|200px]]<br /><applet load="2ckz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ckz, resolution 3.2Å" /> | caption="2ckz, resolution 3.2Å" /> | ||
'''X-RAY STRUCTURE OF RNA POLYMERASE III SUBCOMPLEX C17-C25.'''<br /> | '''X-RAY STRUCTURE OF RNA POLYMERASE III SUBCOMPLEX C17-C25.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a | + | We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a homology model of the nine subunit core enzyme with a new X-ray structure of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved active center for RNA synthesis but a structurally different upstream face for specific initiation complex assembly during promoter selection. The Pol III upstream face includes a HRDC domain in subunit C17 that is translated by 35 A and rotated by 150 degrees compared to its Pol II counterpart. The HRDC domain is essential in vivo, folds independently in vitro, and, unlike other HRDC domains, shows no indication of nucleic acid binding. Thus, the HRDC domain is a functional module that could account for the role of C17 in Pol III promoter-specific initiation. During elongation, C17/25 may bind Pol III transcripts emerging from the adjacent exit pore, because the subcomplex binds to tRNA in vitro. |
==About this Structure== | ==About this Structure== | ||
| - | 2CKZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | + | 2CKZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Armache, K | + | [[Category: Armache, K J.]] |
[[Category: Cramer, P.]] | [[Category: Cramer, P.]] | ||
| - | [[Category: Jansen, R | + | [[Category: Jansen, R P.]] |
| - | [[Category: Jasiak, A | + | [[Category: Jasiak, A J.]] |
[[Category: Martens, B.]] | [[Category: Martens, B.]] | ||
[[Category: class iii gene transcription]] | [[Category: class iii gene transcription]] | ||
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[[Category: trna synthesis]] | [[Category: trna synthesis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:45 2008'' |
Revision as of 14:49, 21 February 2008
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X-RAY STRUCTURE OF RNA POLYMERASE III SUBCOMPLEX C17-C25.
Overview
We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a homology model of the nine subunit core enzyme with a new X-ray structure of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved active center for RNA synthesis but a structurally different upstream face for specific initiation complex assembly during promoter selection. The Pol III upstream face includes a HRDC domain in subunit C17 that is translated by 35 A and rotated by 150 degrees compared to its Pol II counterpart. The HRDC domain is essential in vivo, folds independently in vitro, and, unlike other HRDC domains, shows no indication of nucleic acid binding. Thus, the HRDC domain is a functional module that could account for the role of C17 in Pol III promoter-specific initiation. During elongation, C17/25 may bind Pol III transcripts emerging from the adjacent exit pore, because the subcomplex binds to tRNA in vitro.
About this Structure
2CKZ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model., Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P, Mol Cell. 2006 Jul 7;23(1):71-81. PMID:16818233
Page seeded by OCA on Thu Feb 21 16:49:45 2008
Categories: DNA-directed RNA polymerase | Protein complex | Saccharomyces cerevisiae | Armache, K J. | Cramer, P. | Jansen, R P. | Jasiak, A J. | Martens, B. | Class iii gene transcription | Dna-directed rna polymerase | Eukaryotic nucleic acid polymerase | Hypothetical protein | Multiprotein complex | Nuclear protein | Nucleotidyltransferase | Transcription | Transferase | Trna synthesis
