2cl7
From Proteopedia
(New page: 200px<br /> <applet load="2cl7" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cl7, resolution 1.25Å" /> '''CRYSTAL STRUCTURE A...) |
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| - | [[Image:2cl7.gif|left|200px]]<br /> | + | [[Image:2cl7.gif|left|200px]]<br /><applet load="2cl7" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2cl7" size=" | + | |
caption="2cl7, resolution 1.25Å" /> | caption="2cl7, resolution 1.25Å" /> | ||
'''CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GTP'''<br /> | '''CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GTP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We present a new design for a fluorescence microspectrophotometer for use | + | We present a new design for a fluorescence microspectrophotometer for use in kinetic crystallography in combination with x-ray diffraction experiments. The FLUMIX device (Fluorescence spectroscopy to monitor intermediates in x-ray crystallography) is built for 0 degrees fluorescence detection, which has several advantages in comparison to a conventional fluorometer with 90 degrees design. Due to the reduced spatial requirements and the need for only one objective, the system is highly versatile, easy to handle, and can be used for many different applications. In combination with a conventional stereomicroscope, fluorescence measurements or reaction initiation can be performed directly in a hanging drop crystallization setup. The FLUMIX device can be combined with most x-ray sources, normally without the need of a specialized mechanical support. As a biological model system, we have used H-Ras p21 with an artificially introduced photo-labile GTP precursor (caged GTP) and a covalently attached fluorophore (IANBD amide). Using the FLUMIX system, detailed information about the state of photolyzed crystals of the modified H-Ras p21 (p21(mod)) could be obtained. Measurements in combination with a synchrotron beamline showed significant fluorescence changes in p21(mod) crystals even within a few seconds of x-ray exposure at 100 K. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2CL7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, XY2 and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2CL7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=XY2:'>XY2</scene> and <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CL7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Goody, R | + | [[Category: Goody, R S.]] |
| - | [[Category: Klink, B | + | [[Category: Klink, B U.]] |
| - | [[Category: Scheidig, A | + | [[Category: Scheidig, A J.]] |
[[Category: GTP]] | [[Category: GTP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: r-caged gtp]] | [[Category: r-caged gtp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:47 2008'' |
Revision as of 14:49, 21 February 2008
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CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GTP
Contents |
Overview
We present a new design for a fluorescence microspectrophotometer for use in kinetic crystallography in combination with x-ray diffraction experiments. The FLUMIX device (Fluorescence spectroscopy to monitor intermediates in x-ray crystallography) is built for 0 degrees fluorescence detection, which has several advantages in comparison to a conventional fluorometer with 90 degrees design. Due to the reduced spatial requirements and the need for only one objective, the system is highly versatile, easy to handle, and can be used for many different applications. In combination with a conventional stereomicroscope, fluorescence measurements or reaction initiation can be performed directly in a hanging drop crystallization setup. The FLUMIX device can be combined with most x-ray sources, normally without the need of a specialized mechanical support. As a biological model system, we have used H-Ras p21 with an artificially introduced photo-labile GTP precursor (caged GTP) and a covalently attached fluorophore (IANBD amide). Using the FLUMIX system, detailed information about the state of photolyzed crystals of the modified H-Ras p21 (p21(mod)) could be obtained. Measurements in combination with a synchrotron beamline showed significant fluorescence changes in p21(mod) crystals even within a few seconds of x-ray exposure at 100 K.
Disease
Known diseases associated with this structure: Bladder cancer, somatic OMIM:[190020], Costello syndrome OMIM:[190020], Thyroid carcinoma, follicular, somatic OMIM:[190020]
About this Structure
2CL7 is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction., Klink BU, Goody RS, Scheidig AJ, Biophys J. 2006 Aug 1;91(3):981-92. Epub 2006 May 12. PMID:16698776
Page seeded by OCA on Thu Feb 21 16:49:47 2008
Categories: Homo sapiens | Single protein | Goody, R S. | Klink, B U. | Scheidig, A J. | GTP | MG | XY2 | Caged gtp | Disease mutation | Fluorescence | Golgi apparatus | Gtp | Gtp-binding | Guanine nucleotide binding protein | Intermediate | Lipoprotein | Membrane | Methylation | Nucleotide binding protein | Nucleotide-binding | Palmitate | Prenylation | Proto-oncogene | R-caged gtp
