2clq
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Apoptosis signal-regulating kinase 1 (ASK1) plays an essential role in | + | Apoptosis signal-regulating kinase 1 (ASK1) plays an essential role in stress and immune response and has been linked to the development of several diseases. Here, we present the structure of the human ASK1 catalytic domain in complex with staurosporine. Analytical ultracentrifugation (AUC) and crystallographic analysis showed that ASK1 forms a tight dimer (K(d) approximately 0.2 microM) interacting in a head-to-tail fashion. We found that the ASK1 phosphorylation motifs differ from known ASK1 phosphorylation sites but correspond well to autophosphorylation sites identified by mass spectrometry. Reporter gene assays showed that all three identified in vitro autophosphorylation sites (Thr813, Thr838, Thr842) regulate ASK1 signaling, but site-directed mutants showed catalytic activities similar to wild-type ASK1, suggesting a regulatory mechanism independent of ASK1 kinase activity. The determined high-resolution structure of ASK1 and identified ATP mimetic inhibitors will provide a first starting point for the further development of selective inhibitors. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Structural and | + | Structural and functional characterization of the human protein kinase ASK1., Bunkoczi G, Salah E, Filippakopoulos P, Fedorov O, Muller S, Sobott F, Parker SA, Zhang H, Min W, Turk BE, Knapp S, Structure. 2007 Oct;15(10):1215-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17937911 17937911] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Mitogen-activated protein kinase kinase kinase]] | [[Category: Mitogen-activated protein kinase kinase kinase]] | ||
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[[Category: Arrowsmith, C.]] | [[Category: Arrowsmith, C.]] | ||
[[Category: Bunkoczi, G.]] | [[Category: Bunkoczi, G.]] | ||
| - | [[Category: Delft, F | + | [[Category: Delft, F Von.]] |
[[Category: Edwards, A.]] | [[Category: Edwards, A.]] | ||
[[Category: Fedorov, O.]] | [[Category: Fedorov, O.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:57 2008'' |
Revision as of 14:49, 21 February 2008
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STRUCTURE OF MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
Overview
Apoptosis signal-regulating kinase 1 (ASK1) plays an essential role in stress and immune response and has been linked to the development of several diseases. Here, we present the structure of the human ASK1 catalytic domain in complex with staurosporine. Analytical ultracentrifugation (AUC) and crystallographic analysis showed that ASK1 forms a tight dimer (K(d) approximately 0.2 microM) interacting in a head-to-tail fashion. We found that the ASK1 phosphorylation motifs differ from known ASK1 phosphorylation sites but correspond well to autophosphorylation sites identified by mass spectrometry. Reporter gene assays showed that all three identified in vitro autophosphorylation sites (Thr813, Thr838, Thr842) regulate ASK1 signaling, but site-directed mutants showed catalytic activities similar to wild-type ASK1, suggesting a regulatory mechanism independent of ASK1 kinase activity. The determined high-resolution structure of ASK1 and identified ATP mimetic inhibitors will provide a first starting point for the further development of selective inhibitors.
About this Structure
2CLQ is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Mitogen-activated protein kinase kinase kinase, with EC number 2.7.11.25 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Structural and functional characterization of the human protein kinase ASK1., Bunkoczi G, Salah E, Filippakopoulos P, Fedorov O, Muller S, Sobott F, Parker SA, Zhang H, Min W, Turk BE, Knapp S, Structure. 2007 Oct;15(10):1215-26. PMID:17937911
Page seeded by OCA on Thu Feb 21 16:49:57 2008
Categories: Homo sapiens | Mitogen-activated protein kinase kinase kinase | Single protein | Arrowsmith, C. | Bunkoczi, G. | Delft, F Von. | Edwards, A. | Fedorov, O. | Gileadi, O. | Knapp, S. | Pike, A. | Salah, E. | Sundstrom, M. | Weigelt, J. | STU | Apoptosis | Atp-binding | Kinase | Magnesium | Map kinase | Metal-binding | Nucleotide-binding | Phosphorylation | Protein kinase | Serine/threonine-protein kinase | Transferase
