2cme
From Proteopedia
(New page: 200px<br /><applet load="2cme" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cme, resolution 2.8Å" /> '''THE CRYSTAL STRUCTURE...) |
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| - | [[Image:2cme.gif|left|200px]]<br /><applet load="2cme" size=" | + | [[Image:2cme.gif|left|200px]]<br /><applet load="2cme" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cme, resolution 2.8Å" /> | caption="2cme, resolution 2.8Å" /> | ||
'''THE CRYSTAL STRUCTURE OF SARS CORONAVIRUS ORF-9B PROTEIN'''<br /> | '''THE CRYSTAL STRUCTURE OF SARS CORONAVIRUS ORF-9B PROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | To achieve the greatest output from their limited genomes, viruses | + | To achieve the greatest output from their limited genomes, viruses frequently make use of alternative open reading frames, in which translation is initiated from a start codon within an existing gene and, being out of frame, gives rise to a distinct protein product. These alternative protein products are, as yet, poorly characterized structurally. Here we report the crystal structure of ORF-9b, an alternative open reading frame within the nucleocapsid (N) gene from the SARS coronavirus. The protein has a novel fold, a dimeric tent-like beta structure with an amphipathic surface, and a central hydrophobic cavity that binds lipid molecules. This cavity is likely to be involved in membrane attachment and, in mammalian cells, ORF-9b associates with intracellular vesicles, consistent with a role in the assembly of the virion. Analysis of ORF-9b and other overlapping genes suggests that they provide snapshots of the early evolution of novel protein folds. |
==About this Structure== | ==About this Structure== | ||
| - | 2CME is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with D10 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2CME is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with <scene name='pdbligand=D10:'>D10</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CME OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Human sars coronavirus]] | [[Category: Human sars coronavirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Aplin, R | + | [[Category: Aplin, R T.]] |
| - | [[Category: Aricescu, A | + | [[Category: Aricescu, A R.]] |
[[Category: Assenberg, R.]] | [[Category: Assenberg, R.]] | ||
| - | [[Category: Gilbert, R | + | [[Category: Gilbert, R J.C.]] |
| - | [[Category: Grimes, J | + | [[Category: Grimes, J M.]] |
[[Category: Meier, C.]] | [[Category: Meier, C.]] | ||
| - | [[Category: Stuart, D | + | [[Category: Stuart, D I.]] |
[[Category: D10]] | [[Category: D10]] | ||
[[Category: alternative open reading frame]] | [[Category: alternative open reading frame]] | ||
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[[Category: virus assembly]] | [[Category: virus assembly]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:50:11 2008'' |
Revision as of 14:50, 21 February 2008
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THE CRYSTAL STRUCTURE OF SARS CORONAVIRUS ORF-9B PROTEIN
Overview
To achieve the greatest output from their limited genomes, viruses frequently make use of alternative open reading frames, in which translation is initiated from a start codon within an existing gene and, being out of frame, gives rise to a distinct protein product. These alternative protein products are, as yet, poorly characterized structurally. Here we report the crystal structure of ORF-9b, an alternative open reading frame within the nucleocapsid (N) gene from the SARS coronavirus. The protein has a novel fold, a dimeric tent-like beta structure with an amphipathic surface, and a central hydrophobic cavity that binds lipid molecules. This cavity is likely to be involved in membrane attachment and, in mammalian cells, ORF-9b associates with intracellular vesicles, consistent with a role in the assembly of the virion. Analysis of ORF-9b and other overlapping genes suggests that they provide snapshots of the early evolution of novel protein folds.
About this Structure
2CME is a Single protein structure of sequence from Human sars coronavirus with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of ORF-9b, a lipid binding protein from the SARS coronavirus., Meier C, Aricescu AR, Assenberg R, Aplin RT, Gilbert RJ, Grimes JM, Stuart DI, Structure. 2006 Jul;14(7):1157-65. PMID:16843897
Page seeded by OCA on Thu Feb 21 16:50:11 2008
