2cvq
From Proteopedia
(New page: 200px<br /><applet load="2cvq" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cvq, resolution 2.08Å" /> '''Crystal structure of...) |
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| - | [[Image:2cvq.gif|left|200px]]<br /><applet load="2cvq" size=" | + | [[Image:2cvq.gif|left|200px]]<br /><applet load="2cvq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cvq, resolution 2.08Å" /> | caption="2cvq, resolution 2.08Å" /> | ||
'''Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH'''<br /> | '''Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH'''<br /> | ||
==Overview== | ==Overview== | ||
| - | For better understanding of the coenzyme specificity in NAD-dependent MDH | + | For better understanding of the coenzyme specificity in NAD-dependent MDH (tMDH) from Thermus flavus AT-62, we determined the crystal structures of tMDH-NADP(H) complex at maximally 1.65 A resolution. The overall structure is almost the same as that of the tMDH-NADH complex. However, NADP(H) binds to tMDH in the reverse orientation, where adenine occupies the position near the catalytic center and nicotinamide is positioned at the adenine binding site of the tMDH-NADH complex. Consistent with this, kinetic analysis of the malate-oxidizing reaction revealed that NADP(+) inhibited tMDH at high concentrations. This has provided the first evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 2CVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with NAP and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] Full crystallographic information is available from [http:// | + | 2CVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=NAP:'>NAP</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CVQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: nad-dependent-mdh nadp+ complex]] | [[Category: nad-dependent-mdh nadp+ complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:52:50 2008'' |
Revision as of 14:52, 21 February 2008
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Crystal structure of NAD(H)-dependent malate dehydrogenase complexed with NADPH
Overview
For better understanding of the coenzyme specificity in NAD-dependent MDH (tMDH) from Thermus flavus AT-62, we determined the crystal structures of tMDH-NADP(H) complex at maximally 1.65 A resolution. The overall structure is almost the same as that of the tMDH-NADH complex. However, NADP(H) binds to tMDH in the reverse orientation, where adenine occupies the position near the catalytic center and nicotinamide is positioned at the adenine binding site of the tMDH-NADH complex. Consistent with this, kinetic analysis of the malate-oxidizing reaction revealed that NADP(+) inhibited tMDH at high concentrations. This has provided the first evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme.
About this Structure
2CVQ is a Single protein structure of sequence from Thermus thermophilus with and as ligands. Active as Malate dehydrogenase, with EC number 1.1.1.37 Full crystallographic information is available from OCA.
Reference
Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H)., Tomita T, Fushinobu S, Kuzuyama T, Nishiyama M, Biochem Biophys Res Commun. 2005 Aug 26;334(2):613-8. PMID:16009341
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