2cw0
From Proteopedia
(New page: 200px<br /><applet load="2cw0" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cw0, resolution 3.3Å" /> '''Crystal structure of ...) |
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| - | [[Image:2cw0.gif|left|200px]]<br /><applet load="2cw0" size=" | + | [[Image:2cw0.gif|left|200px]]<br /><applet load="2cw0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cw0, resolution 3.3Å" /> | caption="2cw0, resolution 3.3Å" /> | ||
'''Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution'''<br /> | '''Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We define the target, mechanism, and structural basis of inhibition of | + | We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents. |
==About this Structure== | ==About this Structure== | ||
| - | 2CW0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | + | 2CW0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CW0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Arnold, E.]] | [[Category: Arnold, E.]] | ||
| - | [[Category: Birktoft, J | + | [[Category: Birktoft, J J.]] |
[[Category: Borukhov, S.]] | [[Category: Borukhov, S.]] | ||
[[Category: Dharia, C.]] | [[Category: Dharia, C.]] | ||
| - | [[Category: Ebright, R | + | [[Category: Ebright, R H.]] |
[[Category: Hudson, B.]] | [[Category: Hudson, B.]] | ||
[[Category: Ismail, S.]] | [[Category: Ismail, S.]] | ||
| - | [[Category: Jr., A | + | [[Category: Jr., A D.Clark.]] |
[[Category: Laptenko, O.]] | [[Category: Laptenko, O.]] | ||
[[Category: Lee, J.]] | [[Category: Lee, J.]] | ||
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[[Category: Mukhopadhyay, J.]] | [[Category: Mukhopadhyay, J.]] | ||
[[Category: Napoli, A.]] | [[Category: Napoli, A.]] | ||
| - | [[Category: Sarafianos, S | + | [[Category: Sarafianos, S G.]] |
[[Category: Sineva, E.]] | [[Category: Sineva, E.]] | ||
[[Category: Tuske, S.]] | [[Category: Tuske, S.]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:52:59 2008'' |
Revision as of 14:53, 21 February 2008
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Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution
Overview
We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents.
About this Structure
2CW0 is a Protein complex structure of sequences from Thermus thermophilus with and as ligands. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Inhibition of bacterial RNA polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation., Tuske S, Sarafianos SG, Wang X, Hudson B, Sineva E, Mukhopadhyay J, Birktoft JJ, Leroy O, Ismail S, Clark AD Jr, Dharia C, Napoli A, Laptenko O, Lee J, Borukhov S, Ebright RH, Arnold E, Cell. 2005 Aug 26;122(4):541-52. PMID:16122422
Page seeded by OCA on Thu Feb 21 16:52:59 2008
Categories: DNA-directed RNA polymerase | Protein complex | Thermus thermophilus | Arnold, E. | Birktoft, J J. | Borukhov, S. | Dharia, C. | Ebright, R H. | Hudson, B. | Ismail, S. | Jr., A D.Clark. | Laptenko, O. | Lee, J. | Leroy, O. | Mukhopadhyay, J. | Napoli, A. | Sarafianos, S G. | Sineva, E. | Tuske, S. | Wang, X. | MG | ZN | Bent-bridge helix | Rna polymerase holoenzyme | Transcription
