2d10
From Proteopedia
(New page: 200px<br /> <applet load="2d10" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d10, resolution 2.50Å" /> '''Crystal structure o...) |
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| - | [[Image:2d10.gif|left|200px]]<br /> | + | [[Image:2d10.gif|left|200px]]<br /><applet load="2d10" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2d10" size=" | + | |
caption="2d10, resolution 2.50Å" /> | caption="2d10, resolution 2.50Å" /> | ||
'''Crystal structure of the Radixin FERM domain complexed with the NHERF-1 C-terminal tail peptide'''<br /> | '''Crystal structure of the Radixin FERM domain complexed with the NHERF-1 C-terminal tail peptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Na+/H+ exchanger regulatory factor (NHERF) is a key adaptor protein | + | The Na+/H+ exchanger regulatory factor (NHERF) is a key adaptor protein involved in the anchoring of ion channels and receptors to the actin cytoskeleton through binding to ERM (ezrin/radixin/moesin) proteins. NHERF binds the FERM domain of ERM proteins, although NHERF has no signature Motif-1 sequence for FERM binding found in adhesion molecules. The crystal structures of the radixin FERM domain complexed with the NHERF-1 and NHERF-2 C-terminal peptides revealed a peptide binding site of the FERM domain specific for the 13 residue motif MDWxxxxx(L/I)Fxx(L/F) (Motif-2), which is distinct from Motif-1. This Motif-2 forms an amphipathic alpha helix for hydrophobic docking to subdomain C of the FERM domain. This docking causes induced-fit conformational changes in subdomain C and affects binding to adhesion molecule peptides, while the two binding sites are not overlapped. Our studies provide structural paradigms for versatile ERM linkages between membrane proteins and the cytoskeleton. |
==About this Structure== | ==About this Structure== | ||
| - | 2D10 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 2D10 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D10 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-peptide complex]] | [[Category: protein-peptide complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:54:16 2008'' |
Revision as of 14:54, 21 February 2008
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Crystal structure of the Radixin FERM domain complexed with the NHERF-1 C-terminal tail peptide
Overview
The Na+/H+ exchanger regulatory factor (NHERF) is a key adaptor protein involved in the anchoring of ion channels and receptors to the actin cytoskeleton through binding to ERM (ezrin/radixin/moesin) proteins. NHERF binds the FERM domain of ERM proteins, although NHERF has no signature Motif-1 sequence for FERM binding found in adhesion molecules. The crystal structures of the radixin FERM domain complexed with the NHERF-1 and NHERF-2 C-terminal peptides revealed a peptide binding site of the FERM domain specific for the 13 residue motif MDWxxxxx(L/I)Fxx(L/F) (Motif-2), which is distinct from Motif-1. This Motif-2 forms an amphipathic alpha helix for hydrophobic docking to subdomain C of the FERM domain. This docking causes induced-fit conformational changes in subdomain C and affects binding to adhesion molecule peptides, while the two binding sites are not overlapped. Our studies provide structural paradigms for versatile ERM linkages between membrane proteins and the cytoskeleton.
About this Structure
2D10 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for NHERF recognition by ERM proteins., Terawaki S, Maesaki R, Hakoshima T, Structure. 2006 Apr;14(4):777-89. PMID:16615918
Page seeded by OCA on Thu Feb 21 16:54:16 2008
