2d1p
From Proteopedia
(New page: 200px<br /><applet load="2d1p" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d1p, resolution 2.15Å" /> '''crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2d1p.gif|left|200px]]<br /><applet load="2d1p" size=" | + | [[Image:2d1p.gif|left|200px]]<br /><applet load="2d1p" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2d1p, resolution 2.15Å" /> | caption="2d1p, resolution 2.15Å" /> | ||
'''crystal structure of heterohexameric TusBCD proteins, which are crucial for the tRNA modification'''<br /> | '''crystal structure of heterohexameric TusBCD proteins, which are crucial for the tRNA modification'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Uridine at wobble position 34 of tRNA(Lys), tRNA(Glu), and tRNA(Gln) is | + | Uridine at wobble position 34 of tRNA(Lys), tRNA(Glu), and tRNA(Gln) is exclusively modified into 2-thiouridine (s2U), which is crucial for both precise codon recognition and recognition by the cognate aminoacyl-tRNA synthetases. Recent Escherichia coli genetic studies revealed that the products of five novel genes, tusABCDE, function in the s2U modification. Here, we solved the 2.15 angstroms crystal structure of the E. coli TusBCD complex, a sulfur transfer mediator, forming a heterohexamer composed of a dimer of the heterotrimer. Structure-based sequence alignment suggested two putative active site Cys residues, Cys79 (in TusC) and Cys78 (in TusD), which are exposed on the hexameric complex. In vivo mutant analyses revealed that only Cys78, in the TusD subunit, participates in sulfur transfer during the s2U modification process. Since the single Cys acts as a catalytic residue, we proposed that TusBCD mediates sulfur relay via a putative persulfide state of the TusD subunit. |
==About this Structure== | ==About this Structure== | ||
| - | 2D1P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2D1P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1P OCA]. |
==Reference== | ==Reference== | ||
| Line 23: | Line 23: | ||
[[Category: trna modification]] | [[Category: trna modification]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:54:28 2008'' |
Revision as of 14:54, 21 February 2008
|
crystal structure of heterohexameric TusBCD proteins, which are crucial for the tRNA modification
Overview
Uridine at wobble position 34 of tRNA(Lys), tRNA(Glu), and tRNA(Gln) is exclusively modified into 2-thiouridine (s2U), which is crucial for both precise codon recognition and recognition by the cognate aminoacyl-tRNA synthetases. Recent Escherichia coli genetic studies revealed that the products of five novel genes, tusABCDE, function in the s2U modification. Here, we solved the 2.15 angstroms crystal structure of the E. coli TusBCD complex, a sulfur transfer mediator, forming a heterohexamer composed of a dimer of the heterotrimer. Structure-based sequence alignment suggested two putative active site Cys residues, Cys79 (in TusC) and Cys78 (in TusD), which are exposed on the hexameric complex. In vivo mutant analyses revealed that only Cys78, in the TusD subunit, participates in sulfur transfer during the s2U modification process. Since the single Cys acts as a catalytic residue, we proposed that TusBCD mediates sulfur relay via a putative persulfide state of the TusD subunit.
About this Structure
2D1P is a Protein complex structure of sequences from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis for sulfur relay to RNA mediated by heterohexameric TusBCD complex., Numata T, Fukai S, Ikeuchi Y, Suzuki T, Nureki O, Structure. 2006 Feb;14(2):357-66. PMID:16472754
Page seeded by OCA on Thu Feb 21 16:54:28 2008
