2d45
From Proteopedia
(New page: 200px<br /><applet load="2d45" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d45, resolution 3.8Å" /> '''Crystal structure of ...) |
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| - | [[Image:2d45.gif|left|200px]]<br /><applet load="2d45" size=" | + | [[Image:2d45.gif|left|200px]]<br /><applet load="2d45" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2d45, resolution 3.8Å" /> | caption="2d45, resolution 3.8Å" /> | ||
'''Crystal structure of the MecI-mecA repressor-operator complex'''<br /> | '''Crystal structure of the MecI-mecA repressor-operator complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The dimeric repressor MecI regulates the mecA gene that encodes the | + | The dimeric repressor MecI regulates the mecA gene that encodes the penicillin-binding protein PBP-2a in methicillin-resistant Staphylococcus aureus (MRSA). MecI is similar to BlaI, the repressor for the blaZ gene of beta-lactamase. MecI and BlaI can bind to both operator DNA sequences. The crystal structure of MecI in complex with the 32 base-pair cognate DNA of mec was determined to 3.8 A resolution. MecI is a homodimer and each monomer consists of a compact N-terminal winged-helix domain, which binds to DNA, and a loosely packed C-terminal helical domain, which intertwines with its counter-monomer. The crystal contains horizontal layers of virtual DNA double helices extending in three directions, which are separated by perpendicular DNA segments. Each DNA segment is bound to two MecI dimers. Similar to the BlaI-mec complex, but unlike the MecI-bla complex, the MecI repressors bind to both sides of the mec DNA dyad that contains four conserved sequences of TACA/TGTA. The results confirm the up-and-down binding to the mec operator, which may account for cooperative effect of the repressor. |
==About this Structure== | ==About this Structure== | ||
| - | 2D45 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http:// | + | 2D45 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D45 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the MecI repressor from Staphylococcus aureus in complex with the cognate DNA operator of mec., Safo MK, Ko TP, Musayev FN, Zhao Q, Wang AH, Archer GL, Acta | + | Structure of the MecI repressor from Staphylococcus aureus in complex with the cognate DNA operator of mec., Safo MK, Ko TP, Musayev FN, Zhao Q, Wang AH, Archer GL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):320-4. Epub 2006 Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16582476 16582476] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
| - | [[Category: Archer, G | + | [[Category: Archer, G L.]] |
| - | [[Category: Ko, T | + | [[Category: Ko, T P.]] |
| - | [[Category: Musayev, F | + | [[Category: Musayev, F N.]] |
| - | [[Category: Safo, M | + | [[Category: Safo, M K.]] |
| - | [[Category: Wang, A | + | [[Category: Wang, A H.J.]] |
[[Category: Zhao, Q.]] | [[Category: Zhao, Q.]] | ||
[[Category: b-lactam]] | [[Category: b-lactam]] | ||
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[[Category: repressor]] | [[Category: repressor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:55:13 2008'' |
Revision as of 14:55, 21 February 2008
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Crystal structure of the MecI-mecA repressor-operator complex
Overview
The dimeric repressor MecI regulates the mecA gene that encodes the penicillin-binding protein PBP-2a in methicillin-resistant Staphylococcus aureus (MRSA). MecI is similar to BlaI, the repressor for the blaZ gene of beta-lactamase. MecI and BlaI can bind to both operator DNA sequences. The crystal structure of MecI in complex with the 32 base-pair cognate DNA of mec was determined to 3.8 A resolution. MecI is a homodimer and each monomer consists of a compact N-terminal winged-helix domain, which binds to DNA, and a loosely packed C-terminal helical domain, which intertwines with its counter-monomer. The crystal contains horizontal layers of virtual DNA double helices extending in three directions, which are separated by perpendicular DNA segments. Each DNA segment is bound to two MecI dimers. Similar to the BlaI-mec complex, but unlike the MecI-bla complex, the MecI repressors bind to both sides of the mec DNA dyad that contains four conserved sequences of TACA/TGTA. The results confirm the up-and-down binding to the mec operator, which may account for cooperative effect of the repressor.
About this Structure
2D45 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Structure of the MecI repressor from Staphylococcus aureus in complex with the cognate DNA operator of mec., Safo MK, Ko TP, Musayev FN, Zhao Q, Wang AH, Archer GL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):320-4. Epub 2006 Mar 25. PMID:16582476
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