2d47
From Proteopedia
(New page: 200px<br /><applet load="2d47" size="350" color="white" frame="true" align="right" spinBox="true" caption="2d47, resolution 2.000Å" /> '''MOLECULAR STRUCTURE...) |
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==Overview== | ==Overview== | ||
| - | We have determined the crystal structure of the dodecamer d(CCCCCGCGGGGG), showing for the first time a complete turn of A-DNA. It has average | + | We have determined the crystal structure of the dodecamer d(CCCCCGCGGGGG), showing for the first time a complete turn of A-DNA. It has average structural parameters similar to those determined in fibres. Nevertheless it shows a considerable local variation in structure which is in part associated with the presence of a bound spermine molecule. We conclude that the local DNA conformation does not only depend on the base sequence, but may be strongly modified upon interaction with other molecules. In particular, the CpG sequence, which is found in hypersensitive regions of the genome, appears to be able to easily change its conformation under external influences. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Huynh-Dinh, T.]] | [[Category: Huynh-Dinh, T.]] | ||
[[Category: Igolen, J.]] | [[Category: Igolen, J.]] | ||
| - | [[Category: Subirana, J | + | [[Category: Subirana, J A.]] |
[[Category: Verdaguer, N.]] | [[Category: Verdaguer, N.]] | ||
[[Category: SPM]] | [[Category: SPM]] | ||
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[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:55:12 2008'' |
Revision as of 14:55, 21 February 2008
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MOLECULAR STRUCTURE OF A COMPLETE TURN OF A-DNA
Overview
We have determined the crystal structure of the dodecamer d(CCCCCGCGGGGG), showing for the first time a complete turn of A-DNA. It has average structural parameters similar to those determined in fibres. Nevertheless it shows a considerable local variation in structure which is in part associated with the presence of a bound spermine molecule. We conclude that the local DNA conformation does not only depend on the base sequence, but may be strongly modified upon interaction with other molecules. In particular, the CpG sequence, which is found in hypersensitive regions of the genome, appears to be able to easily change its conformation under external influences.
About this Structure
2D47 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Molecular structure of a complete turn of A-DNA., Verdaguer N, Aymami J, Fernandez-Forner D, Fita I, Coll M, Huynh-Dinh T, Igolen J, Subirana JA, J Mol Biol. 1991 Sep 20;221(2):623-35. PMID:1920437
Page seeded by OCA on Thu Feb 21 16:55:12 2008
