2d94
From Proteopedia
(New page: 200px<br /><applet load="2d94" size="350" color="white" frame="true" align="right" spinBox="true" caption="2d94, resolution 1.700Å" /> '''THE CONFORMATION OF...) |
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==Overview== | ==Overview== | ||
| - | Studies of the crystal structures of more than 30 synthetic DNA fragments | + | Studies of the crystal structures of more than 30 synthetic DNA fragments have provided structural information about three basic forms of the double helix: A-, B- and Z-form DNA. These studies have demonstrated that the DNA double helix adopts a highly variable structure which is related to its base sequence. The extent to which such observed structures are influenced by the crystalline environment can be found by studying the same molecule in different crystalline forms. We have recently crystallized one particular oligomer in various crystal forms. Here we report the results of structural analyses of the different crystal structures and demonstrate that the DNA double helix can adopt a range of conformations in the crystalline state depending on hydration, molecular packing and temperature. These results have implications on our understanding of the influence of the environment on DNA structure, and on the modes of DNA recognition by proteins. |
==About this Structure== | ==About this Structure== | ||
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[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:56:22 2008'' |
Revision as of 14:56, 21 February 2008
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THE CONFORMATION OF THE DNA DOUBLE HELIX IN THE CRYSTAL IS DEPENDENT ON ITS ENVIRONMENT
Overview
Studies of the crystal structures of more than 30 synthetic DNA fragments have provided structural information about three basic forms of the double helix: A-, B- and Z-form DNA. These studies have demonstrated that the DNA double helix adopts a highly variable structure which is related to its base sequence. The extent to which such observed structures are influenced by the crystalline environment can be found by studying the same molecule in different crystalline forms. We have recently crystallized one particular oligomer in various crystal forms. Here we report the results of structural analyses of the different crystal structures and demonstrate that the DNA double helix can adopt a range of conformations in the crystalline state depending on hydration, molecular packing and temperature. These results have implications on our understanding of the influence of the environment on DNA structure, and on the modes of DNA recognition by proteins.
About this Structure
2D94 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The conformation of the DNA double helix in the crystal is dependent on its environment., Shakked Z, Guerstein-Guzikevich G, Eisenstein M, Frolow F, Rabinovich D, Nature. 1989 Nov 23;342(6248):456-60. PMID:2586615
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