2dep
From Proteopedia
(New page: 200px<br /><applet load="2dep" size="450" color="white" frame="true" align="right" spinBox="true" caption="2dep, resolution 1.80Å" /> '''Crystal Structure of...) |
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| - | [[Image:2dep.jpg|left|200px]]<br /><applet load="2dep" size=" | + | [[Image:2dep.jpg|left|200px]]<br /><applet load="2dep" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2dep, resolution 1.80Å" /> | caption="2dep, resolution 1.80Å" /> | ||
'''Crystal Structure of xylanase B from Clostridium stercorarium F9'''<br /> | '''Crystal Structure of xylanase B from Clostridium stercorarium F9'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Recombinant mature xylanase B from Clostridium stercorarium has been | + | Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution. |
==About this Structure== | ==About this Structure== | ||
| - | 2DEP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http:// | + | 2DEP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: xylan degradation]] | [[Category: xylan degradation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:58:02 2008'' |
Revision as of 14:58, 21 February 2008
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Crystal Structure of xylanase B from Clostridium stercorarium F9
Overview
Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.
About this Structure
2DEP is a Single protein structure of sequence from Clostridium stercorarium. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium., Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:14747719
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