2c35
From Proteopedia
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{{STRUCTURE_2c35| PDB=2c35 | SCENE= }} | {{STRUCTURE_2c35| PDB=2c35 | SCENE= }} | ||
| + | ===Subunits Rpb4 and Rpb7 of human RNA polymerase II=== | ||
| + | {{ABSTRACT_PUBMED_16282592}} | ||
| - | === | + | ==Function== |
| - | + | [[http://www.uniprot.org/uniprot/RPB7_HUMAN RPB7_HUMAN]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.<ref>PMID:9852112</ref> | |
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==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:016282592</ref><references group="xtra"/> | + | <ref group="xtra">PMID:016282592</ref><references group="xtra"/><references/> |
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: Rna polymerase ii]] | [[Category: Rna polymerase ii]] | ||
[[Category: Transcription]] | [[Category: Transcription]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 07:43, 27 March 2013
Contents |
Subunits Rpb4 and Rpb7 of human RNA polymerase II
Template:ABSTRACT PUBMED 16282592
Function
[RPB7_HUMAN] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.[1]
About this Structure
2c35 is a 8 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
See Also
Reference
- Meka H, Werner F, Cordell SC, Onesti S, Brick P. Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II. Nucleic Acids Res. 2005 Nov 10;33(19):6435-44. Print 2005. PMID:16282592 doi:http://dx.doi.org/33/19/6435
- ↑ Kershnar E, Wu SY, Chiang CM. Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes. J Biol Chem. 1998 Dec 18;273(51):34444-53. PMID:9852112
