2djw
From Proteopedia
(New page: 200px<br /><applet load="2djw" size="350" color="white" frame="true" align="right" spinBox="true" caption="2djw, resolution 2.40Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | The stand-alone RAM (regulation of amino-acid metabolism) domain protein | + | The stand-alone RAM (regulation of amino-acid metabolism) domain protein SraA from Thermus thermophilus HB8 (TTHA0845) was crystallized in the presence of zinc ions. The X-ray crystal structure was determined using a multiple-wavelength anomalous dispersion technique and was refined at 2.4 A resolution to a final R factor of 25.0%. The monomeric structure is a betaalphabetabetaalphabeta fold and it dimerizes mainly through interactions between the antiparallel beta-sheets. Furthermore, five SraA dimers form a ring with external and internal diameters of 70 and 20 A, respectively. This decameric structure is unique compared with the octameric and dodecameric structures found for other stand-alone RAM-domain proteins and the C-terminal RAM domains of Lrp/AsnC-family proteins. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Structure of the stand-alone RAM-domain protein from Thermus thermophilus HB8., Nakano N, Okazaki N, Satoh S, Takio K, Kuramitsu S, Shinkai A, Yokoyama S, Acta | + | Structure of the stand-alone RAM-domain protein from Thermus thermophilus HB8., Nakano N, Okazaki N, Satoh S, Takio K, Kuramitsu S, Shinkai A, Yokoyama S, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):855-60. Epub 2006 Aug 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16946463 16946463] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Nakano, N.]] | [[Category: Nakano, N.]] | ||
[[Category: Okazaki, N.]] | [[Category: Okazaki, N.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Shinkai, A.]] | [[Category: Shinkai, A.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
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[[Category: thermus thermophilus hb8]] | [[Category: thermus thermophilus hb8]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:59:38 2008'' |
Revision as of 14:59, 21 February 2008
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Crystal structure of TTHA0845 from Thermus thermophilus HB8
Overview
The stand-alone RAM (regulation of amino-acid metabolism) domain protein SraA from Thermus thermophilus HB8 (TTHA0845) was crystallized in the presence of zinc ions. The X-ray crystal structure was determined using a multiple-wavelength anomalous dispersion technique and was refined at 2.4 A resolution to a final R factor of 25.0%. The monomeric structure is a betaalphabetabetaalphabeta fold and it dimerizes mainly through interactions between the antiparallel beta-sheets. Furthermore, five SraA dimers form a ring with external and internal diameters of 70 and 20 A, respectively. This decameric structure is unique compared with the octameric and dodecameric structures found for other stand-alone RAM-domain proteins and the C-terminal RAM domains of Lrp/AsnC-family proteins.
About this Structure
2DJW is a Single protein structure of sequence from Thermus thermophilus with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the stand-alone RAM-domain protein from Thermus thermophilus HB8., Nakano N, Okazaki N, Satoh S, Takio K, Kuramitsu S, Shinkai A, Yokoyama S, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):855-60. Epub 2006 Aug 26. PMID:16946463
Page seeded by OCA on Thu Feb 21 16:59:38 2008
Categories: Single protein | Thermus thermophilus | Nakano, N. | Okazaki, N. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Shinkai, A. | Yokoyama, S. | ZN | National project on protein structural and functional analyses | Nppsfa | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics | Thermus thermophilus hb8
