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3isz
From Proteopedia
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===Crystal structure of mono-zinc form of succinyl-diaminopimelate desuccinylase from Haemophilus influenzae=== | ===Crystal structure of mono-zinc form of succinyl-diaminopimelate desuccinylase from Haemophilus influenzae=== | ||
| + | {{ABSTRACT_PUBMED_20138056}} | ||
| - | + | ==Function== | |
| - | + | [[http://www.uniprot.org/uniprot/DAPE_HAEIN DAPE_HAEIN]] Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls. It can only hydrolyze L,L-N-succinyl-diaminopimelic acid (L,L-SDAP) and is inactive toward D,L-, L,D-, and D,D-SDAP.<ref>PMID:12962500</ref><ref>PMID:16421726</ref><ref>PMID:18712420</ref> | |
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==About this Structure== | ==About this Structure== | ||
| - | + | [[3isz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ISZ OCA]. | |
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID: | + | <ref group="xtra">PMID:020138056</ref><references group="xtra"/><references/> |
| - | [[Category: Haemophilus influenzae | + | [[Category: Haemophilus influenzae]] |
[[Category: Succinyl-diaminopimelate desuccinylase]] | [[Category: Succinyl-diaminopimelate desuccinylase]] | ||
[[Category: Gillner, D M.]] | [[Category: Gillner, D M.]] | ||
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[[Category: Structural genomic]] | [[Category: Structural genomic]] | ||
[[Category: Succinyl-diaminopimelate desuccinylase]] | [[Category: Succinyl-diaminopimelate desuccinylase]] | ||
| - | [[Category: Zinc]] | ||
[[Category: Zn-binding]] | [[Category: Zn-binding]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 7 10:01:46 2010'' | ||
Revision as of 09:31, 27 March 2013
Contents |
Crystal structure of mono-zinc form of succinyl-diaminopimelate desuccinylase from Haemophilus influenzae
Template:ABSTRACT PUBMED 20138056
Function
[DAPE_HAEIN] Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls. It can only hydrolyze L,L-N-succinyl-diaminopimelic acid (L,L-SDAP) and is inactive toward D,L-, L,D-, and D,D-SDAP.[1][2][3]
About this Structure
3isz is a 2 chain structure with sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.
Reference
- Nocek BP, Gillner DM, Fan Y, Holz RC, Joachimiak A. Structural basis for catalysis by the mono- and dimetalated forms of the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase. J Mol Biol. 2010 Apr 2;397(3):617-26. Epub 2010 Feb 4. PMID:20138056 doi:10.1016/j.jmb.2010.01.062
- ↑ Bienvenue DL, Gilner DM, Davis RS, Bennett B, Holz RC. Substrate specificity, metal binding properties, and spectroscopic characterization of the DapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae. Biochemistry. 2003 Sep 16;42(36):10756-63. PMID:12962500 doi:http://dx.doi.org/10.1021/bi034845+
- ↑ Davis R, Bienvenue D, Swierczek SI, Gilner DM, Rajagopal L, Bennett B, Holz RC. Kinetic and spectroscopic characterization of the E134A- and E134D-altered dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae. J Biol Inorg Chem. 2006 Mar;11(2):206-16. Epub 2006 Jan 19. PMID:16421726 doi:10.1007/s00775-005-0071-8
- ↑ Gillner DM, Bienvenue DL, Nocek BP, Joachimiak A, Zachary V, Bennett B, Holz RC. The dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae contains two active-site histidine residues. J Biol Inorg Chem. 2009 Jan;14(1):1-10. doi: 10.1007/s00775-008-0418-z. Epub 2008, Aug 19. PMID:18712420 doi:10.1007/s00775-008-0418-z
Categories: Haemophilus influenzae | Succinyl-diaminopimelate desuccinylase | Gillner, D M. | Holz, R C. | Joachimiak, A. | MCSG, Midwest Center for Structural Genomics. | Nocek, B P. | Amino-acid biosynthesis | Cobalt | Dape | Diaminopimelate biosynthesis | Hydrolase | Lysine biosynthesis | Mcsg | Metal-binding | Metallopeptidase | Midwest center for structural genomic | Protein structure initiative | Psi-2 | Structural genomic | Zn-binding
