2dpk
From Proteopedia
(New page: 200px<br /><applet load="2dpk" size="450" color="white" frame="true" align="right" spinBox="true" caption="2dpk, resolution 2.50Å" /> '''The Crystal Structur...) |
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| - | [[Image:2dpk.gif|left|200px]]<br /><applet load="2dpk" size=" | + | [[Image:2dpk.gif|left|200px]]<br /><applet load="2dpk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2dpk, resolution 2.50Å" /> | caption="2dpk, resolution 2.50Å" /> | ||
'''The Crystal Structure of the Primary Ca2+ Sensor of the Na+/Ca2+ Exchanger'''<br /> | '''The Crystal Structure of the Primary Ca2+ Sensor of the Na+/Ca2+ Exchanger'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Na+/Ca2+ exchanger is a plasma membrane protein that regulates | + | The Na+/Ca2+ exchanger is a plasma membrane protein that regulates intracellular Ca2+ levels in cardiac myocytes. Transport activity is governed by Ca2+, and the primary Ca2+ sensor (CBD1) is located in a large cytoplasmic loop connecting two transmembrane helices. The binding of Ca2+ to the CBD1 sensory domain results in conformational changes that stimulate the exchanger to extrude Ca2+. Here, we present a crystal structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding site consisting of four Ca2+ ions arranged in a tight planar cluster. This intricate coordination pattern for a Ca2+ binding cluster is indicative of a highly sensitive Ca2+ sensor and may represent a general platform for Ca2+ sensing. |
==About this Structure== | ==About this Structure== | ||
| - | 2DPK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with CA, GAI and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2DPK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=GAI:'>GAI</scene> and <scene name='pdbligand=EPE:'>EPE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: cis-proline]] | [[Category: cis-proline]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:01:11 2008'' |
Revision as of 15:01, 21 February 2008
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The Crystal Structure of the Primary Ca2+ Sensor of the Na+/Ca2+ Exchanger
Overview
The Na+/Ca2+ exchanger is a plasma membrane protein that regulates intracellular Ca2+ levels in cardiac myocytes. Transport activity is governed by Ca2+, and the primary Ca2+ sensor (CBD1) is located in a large cytoplasmic loop connecting two transmembrane helices. The binding of Ca2+ to the CBD1 sensory domain results in conformational changes that stimulate the exchanger to extrude Ca2+. Here, we present a crystal structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding site consisting of four Ca2+ ions arranged in a tight planar cluster. This intricate coordination pattern for a Ca2+ binding cluster is indicative of a highly sensitive Ca2+ sensor and may represent a general platform for Ca2+ sensing.
About this Structure
2DPK is a Single protein structure of sequence from Canis lupus familiaris with , and as ligands. Full crystallographic information is available from OCA.
Reference
The crystal structure of the primary Ca2+ sensor of the Na+/Ca2+ exchanger reveals a novel Ca2+ binding motif., Nicoll DA, Sawaya MR, Kwon S, Cascio D, Philipson KD, Abramson J, J Biol Chem. 2006 Aug 4;281(31):21577-81. Epub 2006 Jun 14. PMID:16774926
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