2dpf
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Curculin isolated from Curculigo latifolia, a plant grown in Malaysia, has | + | Curculin isolated from Curculigo latifolia, a plant grown in Malaysia, has an intriguing property of modifying sour taste into sweet taste. In addition to this taste-modifying activity, curculin itself elicits a sweet taste. Although these activities have been attributed to the heterodimeric isoform and not homodimers of curculin, the underlying mechanisms for the dual action of this protein have been largely unknown. To identify critical sites for these activities, we performed a mutational and structural study of recombinant curculin. Based on the comparison of crystal structures of curculin homo- and heterodimers, a series of mutants was designed and subjected to tasting assays. Mapping of amino acid residues on the three-dimensional structure according to their mutational effects revealed that the curculin heterodimer exhibits sweet-tasting and taste-modifying activities through its partially overlapping but distinct molecular surfaces. These findings suggest that the two activities of the curculin heterodimer are expressed through its two different modes of interactions with the T1R2-T1R3 heterodimeric sweet taste receptor. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Curculin | + | Curculin exhibits sweet-tasting and taste-modifying activities through its distinct molecular surfaces., Kurimoto E, Suzuki M, Amemiya E, Yamaguchi Y, Nirasawa S, Shimba N, Xu N, Kashiwagi T, Kawai M, Suzuki E, Kato K, J Biol Chem. 2007 Nov 16;282(46):33252-6. Epub 2007 Sep 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17895249 17895249] |
[[Category: Curculigo latifolia]] | [[Category: Curculigo latifolia]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: taste modifying]] | [[Category: taste modifying]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:01:14 2008'' |
Revision as of 15:01, 21 February 2008
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Crystal Structure of curculin1 homodimer
Overview
Curculin isolated from Curculigo latifolia, a plant grown in Malaysia, has an intriguing property of modifying sour taste into sweet taste. In addition to this taste-modifying activity, curculin itself elicits a sweet taste. Although these activities have been attributed to the heterodimeric isoform and not homodimers of curculin, the underlying mechanisms for the dual action of this protein have been largely unknown. To identify critical sites for these activities, we performed a mutational and structural study of recombinant curculin. Based on the comparison of crystal structures of curculin homo- and heterodimers, a series of mutants was designed and subjected to tasting assays. Mapping of amino acid residues on the three-dimensional structure according to their mutational effects revealed that the curculin heterodimer exhibits sweet-tasting and taste-modifying activities through its partially overlapping but distinct molecular surfaces. These findings suggest that the two activities of the curculin heterodimer are expressed through its two different modes of interactions with the T1R2-T1R3 heterodimeric sweet taste receptor.
About this Structure
2DPF is a Single protein structure of sequence from Curculigo latifolia with as ligand. Full crystallographic information is available from OCA.
Reference
Curculin exhibits sweet-tasting and taste-modifying activities through its distinct molecular surfaces., Kurimoto E, Suzuki M, Amemiya E, Yamaguchi Y, Nirasawa S, Shimba N, Xu N, Kashiwagi T, Kawai M, Suzuki E, Kato K, J Biol Chem. 2007 Nov 16;282(46):33252-6. Epub 2007 Sep 25. PMID:17895249
Page seeded by OCA on Thu Feb 21 17:01:14 2008
Categories: Curculigo latifolia | Single protein | Amemiya, E. | Kashiwagi, T. | Kato, K. | Kawai, M. | Kurimoto, E. | Nirasawa, S. | Shimba, N. | Suzuki, E. | Suzuki, M. | Xu, N. | Yamaguchi, Y. | SO4 | Plant protein | Sweet taste | Taste modifying
