2dyr
From Proteopedia
(New page: 200px<br /><applet load="2dyr" size="450" color="white" frame="true" align="right" spinBox="true" caption="2dyr, resolution 1.80Å" /> '''Bovine heart cytochr...) |
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| - | [[Image:2dyr.jpg|left|200px]]<br /><applet load="2dyr" size=" | + | [[Image:2dyr.jpg|left|200px]]<br /><applet load="2dyr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2dyr, resolution 1.80Å" /> | caption="2dyr, resolution 1.80Å" /> | ||
'''Bovine heart cytochrome C oxidase at the fully oxidized state'''<br /> | '''Bovine heart cytochrome C oxidase at the fully oxidized state'''<br /> | ||
==Overview== | ==Overview== | ||
| - | All 13 lipids, including two cardiolipins, one phosphatidylcholine, three | + | All 13 lipids, including two cardiolipins, one phosphatidylcholine, three phosphatidylethanolamines, four phosphatidylglycerols and three triglycerides, were identified in a crystalline bovine heart cytochrome c oxidase (CcO) preparation. The chain lengths and unsaturated bond positions of the fatty acid moieties determined by mass spectrometry suggest that each lipid head group identifies its specific binding site within CcOs. The X-ray structure demonstrates that the flexibility of the fatty acid tails facilitates their effective space-filling functions and that the four phospholipids stabilize the CcO dimer. Binding of dicyclohexylcarbodiimide to the O(2) transfer pathway of CcO causes two palmitate tails of phosphatidylglycerols to block the pathway, suggesting that the palmitates control the O(2) transfer process.The phosphatidylglycerol with vaccenate (cis-Delta(11)-octadecenoate) was found in CcOs of bovine and Paracoccus denitrificans, the ancestor of mitochondrion, indicating that the vaccenate is conserved in bovine CcO in spite of the abundance of oleate (cis-Delta(9)-octadecenoate). The X-ray structure indicates that the protein moiety selects cis-vaccenate near the O(2) transfer pathway against trans-vaccenate. These results suggest that vaccenate plays a critical role in the O(2) transfer mechanism. |
==About this Structure== | ==About this Structure== | ||
| - | 2DYR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with DMU, CU, ZN, MG, NA, HEA, CUA, UNX, TGL, PGV, CHD, PSC, PEK and CDL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytochrome-c_oxidase Cytochrome-c oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.1 1.9.3.1] Full crystallographic information is available from [http:// | + | 2DYR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=DMU:'>DMU</scene>, <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=HEA:'>HEA</scene>, <scene name='pdbligand=CUA:'>CUA</scene>, <scene name='pdbligand=UNX:'>UNX</scene>, <scene name='pdbligand=TGL:'>TGL</scene>, <scene name='pdbligand=PGV:'>PGV</scene>, <scene name='pdbligand=CHD:'>CHD</scene>, <scene name='pdbligand=PSC:'>PSC</scene>, <scene name='pdbligand=PEK:'>PEK</scene> and <scene name='pdbligand=CDL:'>CDL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytochrome-c_oxidase Cytochrome-c oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.1 1.9.3.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DYR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:04:11 2008'' |
Revision as of 15:04, 21 February 2008
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Bovine heart cytochrome C oxidase at the fully oxidized state
Overview
All 13 lipids, including two cardiolipins, one phosphatidylcholine, three phosphatidylethanolamines, four phosphatidylglycerols and three triglycerides, were identified in a crystalline bovine heart cytochrome c oxidase (CcO) preparation. The chain lengths and unsaturated bond positions of the fatty acid moieties determined by mass spectrometry suggest that each lipid head group identifies its specific binding site within CcOs. The X-ray structure demonstrates that the flexibility of the fatty acid tails facilitates their effective space-filling functions and that the four phospholipids stabilize the CcO dimer. Binding of dicyclohexylcarbodiimide to the O(2) transfer pathway of CcO causes two palmitate tails of phosphatidylglycerols to block the pathway, suggesting that the palmitates control the O(2) transfer process.The phosphatidylglycerol with vaccenate (cis-Delta(11)-octadecenoate) was found in CcOs of bovine and Paracoccus denitrificans, the ancestor of mitochondrion, indicating that the vaccenate is conserved in bovine CcO in spite of the abundance of oleate (cis-Delta(9)-octadecenoate). The X-ray structure indicates that the protein moiety selects cis-vaccenate near the O(2) transfer pathway against trans-vaccenate. These results suggest that vaccenate plays a critical role in the O(2) transfer mechanism.
About this Structure
2DYR is a Protein complex structure of sequences from Bos taurus with , , , , , , , , , , , , and as ligands. Active as Cytochrome-c oxidase, with EC number 1.9.3.1 Full crystallographic information is available from OCA.
Reference
Structures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase., Shinzawa-Itoh K, Aoyama H, Muramoto K, Terada H, Kurauchi T, Tadehara Y, Yamasaki A, Sugimura T, Kurono S, Tsujimoto K, Mizushima T, Yamashita E, Tsukihara T, Yoshikawa S, EMBO J. 2007 Mar 21;26(6):1713-25. Epub 2007 Mar 1. PMID:17332748
Page seeded by OCA on Thu Feb 21 17:04:11 2008
Categories: Bos taurus | Cytochrome-c oxidase | Protein complex | Aoyama, H. | Kurauchi, T. | Mizushima, T. | Muramoto, K. | Shinzawa-Itoh, K. | Tsukihara, T. | Yamashita, E. | Yoshikawa, S. | CDL | CHD | CU | CUA | DMU | HEA | MG | NA | PEK | PGV | PSC | TGL | UNX | ZN | Oxidoreductase
