2e2h
From Proteopedia
(New page: 200px<br /><applet load="2e2h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2e2h, resolution 3.95Å" /> '''RNA polymerase II el...) |
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| - | [[Image:2e2h.gif|left|200px]]<br /><applet load="2e2h" size=" | + | [[Image:2e2h.gif|left|200px]]<br /><applet load="2e2h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2e2h, resolution 3.95Å" /> | caption="2e2h, resolution 3.95Å" /> | ||
'''RNA polymerase II elongation complex at 5 mM Mg2+ with GTP'''<br /> | '''RNA polymerase II elongation complex at 5 mM Mg2+ with GTP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | New structures of RNA polymerase II (pol II) transcribing complexes reveal | + | New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription. |
==About this Structure== | ==About this Structure== | ||
| - | 2E2H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN, MG and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | + | 2E2H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E2H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Bushnell, D | + | [[Category: Bushnell, D A.]] |
| - | [[Category: Kaplan, C | + | [[Category: Kaplan, C D.]] |
| - | [[Category: Kornberg, R | + | [[Category: Kornberg, R D.]] |
[[Category: Wang, D.]] | [[Category: Wang, D.]] | ||
| - | [[Category: Westover, K | + | [[Category: Westover, K D.]] |
[[Category: GTP]] | [[Category: GTP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:05:17 2008'' |
Revision as of 15:05, 21 February 2008
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RNA polymerase II elongation complex at 5 mM Mg2+ with GTP
Overview
New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.
About this Structure
2E2H is a Protein complex structure of sequences from Saccharomyces cerevisiae with , and as ligands. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis., Wang D, Bushnell DA, Westover KD, Kaplan CD, Kornberg RD, Cell. 2006 Dec 1;127(5):941-54. PMID:17129781
Page seeded by OCA on Thu Feb 21 17:05:17 2008
