2e40
From Proteopedia
(New page: 200px<br /><applet load="2e40" size="350" color="white" frame="true" align="right" spinBox="true" caption="2e40, resolution 1.90Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | The white-rot fungus Phanerochaete chrysosporium has two intracellular | + | The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconolactone complexed forms. The overall structure and the characteristic of subsite -1 (glycone site) were similar to those of other known GH1 enzymes. The loop regions covering on the (beta/alpha)(8) barrel was significantly deviated, and they form a unique subsite +1 (aglycone site) of BGL1A. |
==About this Structure== | ==About this Structure== | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:05:46 2008'' |
Revision as of 15:05, 21 February 2008
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Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium in complex with gluconolactone
Overview
The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconolactone complexed forms. The overall structure and the characteristic of subsite -1 (glycone site) were similar to those of other known GH1 enzymes. The loop regions covering on the (beta/alpha)(8) barrel was significantly deviated, and they form a unique subsite +1 (aglycone site) of BGL1A.
About this Structure
2E40 is a Single protein structure of sequence from Phanerochaete chrysosporium with as ligand. Active as Beta-glucosidase, with EC number 3.2.1.21 Full crystallographic information is available from OCA.
Reference
Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium., Nijikken Y, Tsukada T, Igarashi K, Samejima M, Wakagi T, Shoun H, Fushinobu S, FEBS Lett. 2007 Apr 3;581(7):1514-20. Epub 2007 Mar 13. PMID:17376440
Page seeded by OCA on Thu Feb 21 17:05:46 2008
Categories: Beta-glucosidase | Phanerochaete chrysosporium | Single protein | Fushinobu, S. | Igarashi, K. | Nijikken, Y. | Samejima, M. | Tsukada, T. | LGC | Clan gh-a | Glycoside hydrolase family 1 | National project on protein structural and functional analyses | Nppsfa | Structural genomics | Tim barrel
