This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3lz9
From Proteopedia
| Line 1: | Line 1: | ||
| - | {{Seed}} | ||
| - | [[Image:3lz9.png|left|200px]] | ||
| - | |||
| - | <!-- | ||
| - | The line below this paragraph, containing "STRUCTURE_3lz9", creates the "Structure Box" on the page. | ||
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet) | ||
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| - | or leave the SCENE parameter empty for the default display. | ||
| - | --> | ||
{{STRUCTURE_3lz9| PDB=3lz9 | SCENE= }} | {{STRUCTURE_3lz9| PDB=3lz9 | SCENE= }} | ||
| - | |||
===The Crystal Structure of 5-epi-aristolochene synthase M4 mutant complexed with (2-trans,6-trans)-2-fluorofarnesyl diphosphate=== | ===The Crystal Structure of 5-epi-aristolochene synthase M4 mutant complexed with (2-trans,6-trans)-2-fluorofarnesyl diphosphate=== | ||
| + | {{ABSTRACT_PUBMED_20175559}} | ||
| - | + | ==Function== | |
| - | + | [[http://www.uniprot.org/uniprot/5EAS_TOBAC 5EAS_TOBAC]] Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic intermediate 5-epi-aristolochene, initial step in the conversion of FPP to the sesquiterpenoid antifungal phytoalexin capsidiol. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce the bicyclic 5-epi-aristolochene. | |
| - | + | ||
| - | + | ||
| - | -- | + | |
| - | + | ||
==About this Structure== | ==About this Structure== | ||
| - | + | [[3lz9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZ9 OCA]. | |
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID: | + | <ref group="xtra">PMID:020175559</ref><references group="xtra"/><references/> |
[[Category: Aristolochene synthase]] | [[Category: Aristolochene synthase]] | ||
[[Category: Nicotiana tabacum]] | [[Category: Nicotiana tabacum]] | ||
| Line 29: | Line 16: | ||
[[Category: Dellas, N.]] | [[Category: Dellas, N.]] | ||
[[Category: Faraldos, J A.]] | [[Category: Faraldos, J A.]] | ||
| - | [[Category: | + | [[Category: Hess, B A.]] |
[[Category: Maille, P E.O.]] | [[Category: Maille, P E.O.]] | ||
[[Category: Noel, J P.]] | [[Category: Noel, J P.]] | ||
| Line 35: | Line 22: | ||
[[Category: Zhao, M.]] | [[Category: Zhao, M.]] | ||
[[Category: 5-epi-aristolochene synthase]] | [[Category: 5-epi-aristolochene synthase]] | ||
| - | [[Category: Cytoplasm]] | ||
[[Category: Lyase]] | [[Category: Lyase]] | ||
[[Category: Magnesium]] | [[Category: Magnesium]] | ||
| Line 41: | Line 27: | ||
[[Category: Metal-binding domain]] | [[Category: Metal-binding domain]] | ||
[[Category: Plant terpenoid cyclase]] | [[Category: Plant terpenoid cyclase]] | ||
| - | |||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 28 11:24:00 2010'' | ||
Revision as of 23:06, 10 April 2013
Contents |
The Crystal Structure of 5-epi-aristolochene synthase M4 mutant complexed with (2-trans,6-trans)-2-fluorofarnesyl diphosphate
Template:ABSTRACT PUBMED 20175559
Function
[5EAS_TOBAC] Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic intermediate 5-epi-aristolochene, initial step in the conversion of FPP to the sesquiterpenoid antifungal phytoalexin capsidiol. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce the bicyclic 5-epi-aristolochene.
About this Structure
3lz9 is a 1 chain structure with sequence from Nicotiana tabacum. Full crystallographic information is available from OCA.
Reference
- Noel JP, Dellas N, Faraldos JA, Zhao M, Hess BA, Smentek L, Coates RM, O'Maille PE. Structural Elucidation of Cisoid and Transoid Cyclization Pathways of a Sesquiterpene Synthase Using 2-Fluorofarnesyl Diphosphates. ACS Chem Biol. 2010 Mar 8. PMID:20175559 doi:10.1021/cb900295g
