2es5
From Proteopedia
(New page: 200px<br /><applet load="2es5" size="350" color="white" frame="true" align="right" spinBox="true" caption="2es5" /> '''Structure of the SRE RNA'''<br /> ==Overvie...) |
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==Overview== | ==Overview== | ||
| - | Although the abundant sterile alpha motif (SAM) domain was originally | + | Although the abundant sterile alpha motif (SAM) domain was originally classified as a protein-protein interaction domain, it has recently been shown that certain SAM domains have the ability to bind RNA, defining a new type of post-transcriptional gene regulator. To further understand the function of SAM-RNA recognition, we determined the solution structures of the SAM domain of the Saccharomyces cerevisiae Vts1p (Vts1p-SAM) and the Smaug response element (SRE) stem-loop RNA as a complex and in isolation. The structures show that Vts1p-SAM recognizes predominantly the shape of the SRE rather than its sequence, with the exception of a G located at the tip of the pentaloop. Using microarray gene profiling, we identified several genes in S. cerevisiae that seem to be regulated by Vts1p and contain one or more copies of the SRE. |
==About this Structure== | ==About this Structure== | ||
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Shape-specific recognition in the structure of the Vts1p SAM domain with RNA., Oberstrass FC, Lee A, Stefl R, Janis M, Chanfreau G, Allain FH, Nat Struct Mol Biol. 2006 Feb;13(2):160-7. Epub 2006 Jan 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16429156 16429156] | Shape-specific recognition in the structure of the Vts1p SAM domain with RNA., Oberstrass FC, Lee A, Stefl R, Janis M, Chanfreau G, Allain FH, Nat Struct Mol Biol. 2006 Feb;13(2):160-7. Epub 2006 Jan 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16429156 16429156] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Allain, F | + | [[Category: Allain, F H.T.]] |
[[Category: pentaloop]] | [[Category: pentaloop]] | ||
[[Category: rna structure]] | [[Category: rna structure]] | ||
[[Category: stem-loop]] | [[Category: stem-loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:14:00 2008'' |
Revision as of 15:14, 21 February 2008
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Structure of the SRE RNA
Overview
Although the abundant sterile alpha motif (SAM) domain was originally classified as a protein-protein interaction domain, it has recently been shown that certain SAM domains have the ability to bind RNA, defining a new type of post-transcriptional gene regulator. To further understand the function of SAM-RNA recognition, we determined the solution structures of the SAM domain of the Saccharomyces cerevisiae Vts1p (Vts1p-SAM) and the Smaug response element (SRE) stem-loop RNA as a complex and in isolation. The structures show that Vts1p-SAM recognizes predominantly the shape of the SRE rather than its sequence, with the exception of a G located at the tip of the pentaloop. Using microarray gene profiling, we identified several genes in S. cerevisiae that seem to be regulated by Vts1p and contain one or more copies of the SRE.
About this Structure
2ES5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Shape-specific recognition in the structure of the Vts1p SAM domain with RNA., Oberstrass FC, Lee A, Stefl R, Janis M, Chanfreau G, Allain FH, Nat Struct Mol Biol. 2006 Feb;13(2):160-7. Epub 2006 Jan 22. PMID:16429156
Page seeded by OCA on Thu Feb 21 17:14:00 2008
