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2es4
From Proteopedia
(New page: 200px<br /><applet load="2es4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2es4, resolution 1.85Å" /> '''Crystal structure of...) |
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| - | [[Image:2es4.gif|left|200px]]<br /><applet load="2es4" size=" | + | [[Image:2es4.gif|left|200px]]<br /><applet load="2es4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2es4, resolution 1.85Å" /> | caption="2es4, resolution 1.85Å" /> | ||
'''Crystal structure of the Burkholderia glumae lipase-specific foldase in complex with its cognate lipase'''<br /> | '''Crystal structure of the Burkholderia glumae lipase-specific foldase in complex with its cognate lipase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Secretion via the type II secretion pathway in Gram-negative bacteria | + | Secretion via the type II secretion pathway in Gram-negative bacteria often relies crucially on steric chaperones in the periplasm. Here, we report the crystal structure of the soluble form of a lipase-specific foldase (Lif) from Burkholderia glumae in complex with its cognate lipase. The structure reveals how Lif uses a novel alpha-helical scaffold to embrace lipase, thereby creating an unusually extensive folding platform. |
==About this Structure== | ==About this Structure== | ||
| - | 2ES4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Burkholderia_glumae Burkholderia glumae] with CA and IOD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http:// | + | 2ES4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Burkholderia_glumae Burkholderia glumae] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=IOD:'>IOD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ES4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Triacylglycerol lipase]] | [[Category: Triacylglycerol lipase]] | ||
| - | [[Category: Gelder, P | + | [[Category: Gelder, P Van.]] |
[[Category: Pauwels, K.]] | [[Category: Pauwels, K.]] | ||
| - | [[Category: Savvides, S | + | [[Category: Savvides, S N.]] |
[[Category: Tommassen, J.]] | [[Category: Tommassen, J.]] | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
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[[Category: triacylglycerol hydrolase]] | [[Category: triacylglycerol hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:14:02 2008'' |
Revision as of 15:14, 21 February 2008
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Crystal structure of the Burkholderia glumae lipase-specific foldase in complex with its cognate lipase
Overview
Secretion via the type II secretion pathway in Gram-negative bacteria often relies crucially on steric chaperones in the periplasm. Here, we report the crystal structure of the soluble form of a lipase-specific foldase (Lif) from Burkholderia glumae in complex with its cognate lipase. The structure reveals how Lif uses a novel alpha-helical scaffold to embrace lipase, thereby creating an unusually extensive folding platform.
About this Structure
2ES4 is a Protein complex structure of sequences from Burkholderia glumae with and as ligands. Active as Triacylglycerol lipase, with EC number 3.1.1.3 Full crystallographic information is available from OCA.
Reference
Structure of a membrane-based steric chaperone in complex with its lipase substrate., Pauwels K, Lustig A, Wyns L, Tommassen J, Savvides SN, Van Gelder P, Nat Struct Mol Biol. 2006 Apr;13(4):374-5. Epub 2006 Mar 5. PMID:16518399
Page seeded by OCA on Thu Feb 21 17:14:02 2008
Categories: Burkholderia glumae | Protein complex | Triacylglycerol lipase | Gelder, P Van. | Pauwels, K. | Savvides, S N. | Tommassen, J. | Wyns, L. | CA | IOD | A/b hydrolase fold | All alpha helix protein | Extensive interaction area | Protein-protein complex | Steric chaperone | Triacylglycerol hydrolase
