2euv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2euv" size="450" color="white" frame="true" align="right" spinBox="true" caption="2euv, resolution 1.940&Aring;" /> '''Principles of prote...)
Line 1: Line 1:
-
[[Image:2euv.gif|left|200px]]<br /><applet load="2euv" size="450" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2euv.gif|left|200px]]<br /><applet load="2euv" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2euv, resolution 1.940&Aring;" />
caption="2euv, resolution 1.940&Aring;" />
'''Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes'''<br />
'''Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes'''<br />
==Overview==
==Overview==
-
The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds, a DNA consensus sequence (the middle sporulation element [MSE]) to, activate gene expression after the successful completion of meiotic, recombination. Here we report the X-ray crystal structures of Ndt80 bound, to ten distinct MSE variants. Comparison of these structures with the, structure of Ndt80 bound to a consensus MSE reveals structural principles, that determine the DNA binding specificity of this transcription factor., The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are, recognized by arginine side chains through a combination of hydrogen, bonding and cation-pi interactions. The 3' AT-rich region is recognized, via minor groove contacts that sterically exclude the N2 atom of GC base, pairs. The conformation of the AT-rich region is fixed by interactions, with the protein that favor recognition of poly(A)-poly(T) versus mixed AT, sequences through an avoidance of major groove steric clashes at 5'-ApT-3', steps.
+
The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps.
==About this Structure==
==About this Structure==
-
2EUV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2EUV OCA].
+
2EUV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EUV OCA].
==Reference==
==Reference==
Line 13: Line 13:
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Single protein]]
-
[[Category: Glover, J.N.]]
+
[[Category: Glover, J N.]]
-
[[Category: Lamoureux, J.S.]]
+
[[Category: Lamoureux, J S.]]
[[Category: beta-barrel]]
[[Category: beta-barrel]]
[[Category: ig-fold transcription factor]]
[[Category: ig-fold transcription factor]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:09:39 2007''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:14:44 2008''

Revision as of 15:14, 21 February 2008

Image:2euv.gif

2euv, resolution 1.940Å

Drag the structure with the mouse to rotate

Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes

Overview

The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps.

About this Structure

2EUV is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:16531239

Page seeded by OCA on Thu Feb 21 17:14:44 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2euv"
Personal tools