2euw
From Proteopedia
(New page: 200px<br /><applet load="2euw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2euw, resolution 1.680Å" /> '''Structure of a Ndt8...) |
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| - | [[Image:2euw.gif|left|200px]]<br /><applet load="2euw" size=" | + | [[Image:2euw.gif|left|200px]]<br /><applet load="2euw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2euw, resolution 1.680Å" /> | caption="2euw, resolution 1.680Å" /> | ||
'''Structure of a Ndt80-DNA complex (MSE mutant mA4T)'''<br /> | '''Structure of a Ndt80-DNA complex (MSE mutant mA4T)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds | + | The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps. |
==About this Structure== | ==About this Structure== | ||
| - | 2EUW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 2EUW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EUW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Glover, J | + | [[Category: Glover, J N.]] |
| - | [[Category: Lamoureux, J | + | [[Category: Lamoureux, J S.]] |
[[Category: beta-barrel]] | [[Category: beta-barrel]] | ||
[[Category: ig-fold transcription factor]] | [[Category: ig-fold transcription factor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:14:45 2008'' |
Revision as of 15:14, 21 February 2008
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Structure of a Ndt80-DNA complex (MSE mutant mA4T)
Overview
The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps.
About this Structure
2EUW is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:16531239
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