2f1n
From Proteopedia
(New page: 200px<br /><applet load="2f1n" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f1n, resolution 1.73Å" /> '''Structure of CdtB, t...) |
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| - | [[Image:2f1n.gif|left|200px]]<br /><applet load="2f1n" size=" | + | [[Image:2f1n.gif|left|200px]]<br /><applet load="2f1n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2f1n, resolution 1.73Å" /> | caption="2f1n, resolution 1.73Å" /> | ||
'''Structure of CdtB, the biologically active subunit of Cytolethal Distending Toxin'''<br /> | '''Structure of CdtB, the biologically active subunit of Cytolethal Distending Toxin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cytolethal distending toxin (CDT) induces cell cycle arrest and apoptosis | + | Cytolethal distending toxin (CDT) induces cell cycle arrest and apoptosis in eukaryotic cells, which are mediated by the DNA-damaging CdtB subunit. Here we report the first x-ray structure of an isolated CdtB subunit (Escherichia coli-II CdtB, EcCdtB). In conjunction with previous structural and biochemical observations, active site structural comparisons between free and holotoxin-assembled CdtBs suggested that CDT intoxication is contingent upon holotoxin disassembly. Solution NMR structural and 15N relaxation studies of free EcCdtB revealed disorder in the interface with the CdtA and CdtC subunits (residues Gly233-Asp242). Residues Leu186-Thr209 of EcCdtB, which encompasses tandem arginine residues essential for nuclear translocation and intoxication, were also disordered in solution. In stark contrast, nearly identical well defined alpha-helix and beta-strand secondary structures were observed in this region of the free and holotoxin CdtB crystallographic models, suggesting that distinct changes in structural ordering characterize subunit disassembly and nuclear localization factor binding functions. |
==About this Structure== | ==About this Structure== | ||
| - | 2F1N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 2F1N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F1N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Dreyfus, L | + | [[Category: Dreyfus, L A.]] |
| - | [[Category: Hontz, J | + | [[Category: Hontz, J S.]] |
| - | [[Category: Yoder, M | + | [[Category: Yoder, M D.]] |
[[Category: cdt]] | [[Category: cdt]] | ||
[[Category: cytolethal distending toxin]] | [[Category: cytolethal distending toxin]] | ||
[[Category: dnase i]] | [[Category: dnase i]] | ||
| - | [[Category: e | + | [[Category: e coli]] |
[[Category: microbatch]] | [[Category: microbatch]] | ||
[[Category: toxin]] | [[Category: toxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:16:52 2008'' |
Revision as of 15:16, 21 February 2008
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Structure of CdtB, the biologically active subunit of Cytolethal Distending Toxin
Overview
Cytolethal distending toxin (CDT) induces cell cycle arrest and apoptosis in eukaryotic cells, which are mediated by the DNA-damaging CdtB subunit. Here we report the first x-ray structure of an isolated CdtB subunit (Escherichia coli-II CdtB, EcCdtB). In conjunction with previous structural and biochemical observations, active site structural comparisons between free and holotoxin-assembled CdtBs suggested that CDT intoxication is contingent upon holotoxin disassembly. Solution NMR structural and 15N relaxation studies of free EcCdtB revealed disorder in the interface with the CdtA and CdtC subunits (residues Gly233-Asp242). Residues Leu186-Thr209 of EcCdtB, which encompasses tandem arginine residues essential for nuclear translocation and intoxication, were also disordered in solution. In stark contrast, nearly identical well defined alpha-helix and beta-strand secondary structures were observed in this region of the free and holotoxin CdtB crystallographic models, suggesting that distinct changes in structural ordering characterize subunit disassembly and nuclear localization factor binding functions.
About this Structure
2F1N is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Differences in crystal and solution structures of the cytolethal distending toxin B subunit: Relevance to nuclear translocation and functional activation., Hontz JS, Villar-Lecumberri MT, Potter BM, Yoder MD, Dreyfus LA, Laity JH, J Biol Chem. 2006 Sep 1;281(35):25365-72. Epub 2006 Jun 28. PMID:16809347
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