2f2h
From Proteopedia
(New page: 200px<br /><applet load="2f2h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f2h, resolution 1.950Å" /> '''Structure of the Yi...) |
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| - | [[Image:2f2h.gif|left|200px]]<br /><applet load="2f2h" size=" | + | [[Image:2f2h.gif|left|200px]]<br /><applet load="2f2h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2f2h, resolution 1.950Å" /> | caption="2f2h, resolution 1.950Å" /> | ||
'''Structure of the YicI thiosugar Michaelis complex'''<br /> | '''Structure of the YicI thiosugar Michaelis complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | For the first time, the thioglycoligase strategy has been successfully applied to -glycosidases. The -thioglycoligases derived from the family 31 glycosidases, -xylosidase from E. coli (YicI) and -glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using -glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 -glycosidases and the molecular basis of an associated genetic disease. | + | For the first time, the thioglycoligase strategy has been successfully applied to alpha-glycosidases. The alpha-thioglycoligases derived from the family 31 glycosidases, alpha-xylosidase from E. coli (YicI) and alpha-glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using alpha-glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 alpha-glycosidases and the molecular basis of an associated genetic disease. |
==About this Structure== | ==About this Structure== | ||
| - | 2F2H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, MPO, XTG and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2F2H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MPO:'>MPO</scene>, <scene name='pdbligand=XTG:'>XTG</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F2H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kim, Y | + | [[Category: Kim, Y W.]] |
| - | [[Category: Lovering, A | + | [[Category: Lovering, A L.]] |
| - | [[Category: Strynadka, N | + | [[Category: Strynadka, N C.J.]] |
| - | [[Category: Withers, S | + | [[Category: Withers, S G.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: MPO]] | [[Category: MPO]] | ||
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[[Category: beta8alpha8 barrel]] | [[Category: beta8alpha8 barrel]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:01 2008'' |
Revision as of 15:17, 21 February 2008
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Structure of the YicI thiosugar Michaelis complex
Overview
For the first time, the thioglycoligase strategy has been successfully applied to alpha-glycosidases. The alpha-thioglycoligases derived from the family 31 glycosidases, alpha-xylosidase from E. coli (YicI) and alpha-glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using alpha-glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 alpha-glycosidases and the molecular basis of an associated genetic disease.
About this Structure
2F2H is a Single protein structure of sequence from Escherichia coli with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex., Kim YW, Lovering AL, Chen H, Kantner T, McIntosh LP, Strynadka NC, Withers SG, J Am Chem Soc. 2006 Feb 22;128(7):2202-3. PMID:16478160
Page seeded by OCA on Thu Feb 21 17:17:01 2008
Categories: Escherichia coli | Single protein | Kim, Y W. | Lovering, A L. | Strynadka, N C.J. | Withers, S G. | GOL | MPO | SO4 | XTG | Beta8alpha8 barrel
