2f31
From Proteopedia
(New page: 200px<br /><applet load="2f31" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f31, resolution 2.10Å" /> '''Crystal structure of...) |
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| - | [[Image:2f31.gif|left|200px]]<br /><applet load="2f31" size=" | + | [[Image:2f31.gif|left|200px]]<br /><applet load="2f31" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2f31, resolution 2.10Å" /> | caption="2f31, resolution 2.10Å" /> | ||
'''Crystal structure of the autoinhibitory switch in Formin mDia1; the DID/DAD complex'''<br /> | '''Crystal structure of the autoinhibitory switch in Formin mDia1; the DID/DAD complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Diaphanous-related formins (DRFs) regulate the nucleation and | + | Diaphanous-related formins (DRFs) regulate the nucleation and polymerization of unbranched actin filaments. The activity of DRFs is inhibited by an intramolecular interaction between their N-terminal regulatory region and a conserved C-terminal segment termed the Diaphanous autoinhibitory domain (DAD). Binding of GTP bound Rho to the mDia1 N terminus releases this autoinhibitory restraint. Here, we describe the crystal structure of the DAD segment of mDia1 in complex with the relevant N-terminal fragment, termed the DID domain. The structure reveals that the DAD segment forms an amphipathic helix that binds a conserved, concave surface on the DID domain. Comparison with the structure of the mDia1 N terminus bound to RhoC suggests that release of the autoinhibitory DAD interaction is accomplished largely by Rho-induced restructuring of the adjacent GTPase binding subdomain (GBD), but also by electrostatic repulsion and a small, direct steric occlusion of the DAD binding cleft by Rho itself. |
==About this Structure== | ==About this Structure== | ||
| - | 2F31 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 2F31 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F31 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Eck, M | + | [[Category: Eck, M J.]] |
| - | [[Category: Nezami, A | + | [[Category: Nezami, A G.]] |
[[Category: Poy, F.]] | [[Category: Poy, F.]] | ||
[[Category: armadillo repeats]] | [[Category: armadillo repeats]] | ||
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[[Category: protein-protein complex]] | [[Category: protein-protein complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:09 2008'' |
Revision as of 15:17, 21 February 2008
|
Crystal structure of the autoinhibitory switch in Formin mDia1; the DID/DAD complex
Overview
Diaphanous-related formins (DRFs) regulate the nucleation and polymerization of unbranched actin filaments. The activity of DRFs is inhibited by an intramolecular interaction between their N-terminal regulatory region and a conserved C-terminal segment termed the Diaphanous autoinhibitory domain (DAD). Binding of GTP bound Rho to the mDia1 N terminus releases this autoinhibitory restraint. Here, we describe the crystal structure of the DAD segment of mDia1 in complex with the relevant N-terminal fragment, termed the DID domain. The structure reveals that the DAD segment forms an amphipathic helix that binds a conserved, concave surface on the DID domain. Comparison with the structure of the mDia1 N terminus bound to RhoC suggests that release of the autoinhibitory DAD interaction is accomplished largely by Rho-induced restructuring of the adjacent GTPase binding subdomain (GBD), but also by electrostatic repulsion and a small, direct steric occlusion of the DAD binding cleft by Rho itself.
About this Structure
2F31 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure of the autoinhibitory switch in formin mDia1., Nezami AG, Poy F, Eck MJ, Structure. 2006 Feb;14(2):257-63. PMID:16472745
Page seeded by OCA on Thu Feb 21 17:17:09 2008
