2f40
From Proteopedia
(New page: 200px<br /><applet load="2f40" size="350" color="white" frame="true" align="right" spinBox="true" caption="2f40" /> '''Structure of a Novel Protein from Backbone-C...) |
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==Overview== | ==Overview== | ||
| - | Targeting of proteins for structure determination in structural genomic | + | Targeting of proteins for structure determination in structural genomic programs often includes the use of threading and fold recognition methods to exclude proteins belonging to well-populated fold families, but such methods can still fail to recognize preexisting folds. The authors illustrate here a method in which limited amounts of structural data are used to improve an initial homology search and the data are subsequently used to produce a structure by data-constrained refinement of an identified structural template. The data used are primarily NMR-based residual dipolar couplings, but they also include additional chemical shift and backbone-nuclear Overhauser effect data. Using this methodology, a backbone structure was efficiently produced for a 10 kDa protein (PF1455) from Pyrococcus furiosus. Its relationship to existing structures and its probable function are discussed. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bansal, S.]] | [[Category: Bansal, S.]] | ||
| - | [[Category: Prestegard, J | + | [[Category: Prestegard, J H.]] |
| - | [[Category: SECSG, Southeast | + | [[Category: SECSG, Southeast Collaboratory for Structural Genomics.]] |
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: protein structure prediction]] | [[Category: protein structure prediction]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:30 2008'' |
Revision as of 15:17, 21 February 2008
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Structure of a Novel Protein from Backbone-Centered NMR Data and NMR-Assisted Structure Prediction
Overview
Targeting of proteins for structure determination in structural genomic programs often includes the use of threading and fold recognition methods to exclude proteins belonging to well-populated fold families, but such methods can still fail to recognize preexisting folds. The authors illustrate here a method in which limited amounts of structural data are used to improve an initial homology search and the data are subsequently used to produce a structure by data-constrained refinement of an identified structural template. The data used are primarily NMR-based residual dipolar couplings, but they also include additional chemical shift and backbone-nuclear Overhauser effect data. Using this methodology, a backbone structure was efficiently produced for a 10 kDa protein (PF1455) from Pyrococcus furiosus. Its relationship to existing structures and its probable function are discussed.
About this Structure
2F40 is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.
Reference
Structure determination of a new protein from backbone-centered NMR data and NMR-assisted structure prediction., Mayer KL, Qu Y, Bansal S, LeBlond PD, Jenney FE Jr, Brereton PS, Adams MW, Xu Y, Prestegard JH, Proteins. 2006 Nov 1;65(2):480-9. PMID:16927360
Page seeded by OCA on Thu Feb 21 17:17:30 2008
Categories: Pyrococcus furiosus | Single protein | Bansal, S. | Prestegard, J H. | SECSG, Southeast Collaboratory for Structural Genomics. | Protein structure initiative | Protein structure prediction | Psi | Pyrococcus furious | Residual dipolar couplings | Secsg | Simulated annealing | Southeast collaboratory for structural genomics | Structural genomics
