2f4y
From Proteopedia
(New page: 200px<br /><applet load="2f4y" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f4y, resolution 2.15Å" /> '''Barnase cross-linked...) |
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| - | [[Image:2f4y.gif|left|200px]]<br /><applet load="2f4y" size=" | + | [[Image:2f4y.gif|left|200px]]<br /><applet load="2f4y" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2f4y, resolution 2.15Å" /> | caption="2f4y, resolution 2.15Å" /> | ||
'''Barnase cross-linked with glutaraldehyde'''<br /> | '''Barnase cross-linked with glutaraldehyde'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Structural data about the early step of protein denaturation were obtained | + | Structural data about the early step of protein denaturation were obtained from cross-linked crystals for two small proteins: barnase and lysozyme. Several denaturant agents like urea, bromoethanol or thiourea were used at increasing concentrations up to a limit leading to crystal disruption (>or=2 to 6 M). Before the complete destruction of the crystal order started, specific binding sites were observed at the protein surfaces, an indication that the preliminary step of denaturation is the disproportion of intermolecular polar bonds to the benefit of the agent "parasiting" the surface. The analysis of the thermal factors first agree with a stabilization effect at low or moderate concentration of denaturants rapidly followed by a destabilization at specific weak points when the number of sites increase (overflooding effect). |
==About this Structure== | ==About this Structure== | ||
| - | 2F4Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2F4Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: urea]] | [[Category: urea]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:42 2008'' |
Revision as of 15:17, 21 February 2008
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Barnase cross-linked with glutaraldehyde
Overview
Structural data about the early step of protein denaturation were obtained from cross-linked crystals for two small proteins: barnase and lysozyme. Several denaturant agents like urea, bromoethanol or thiourea were used at increasing concentrations up to a limit leading to crystal disruption (>or=2 to 6 M). Before the complete destruction of the crystal order started, specific binding sites were observed at the protein surfaces, an indication that the preliminary step of denaturation is the disproportion of intermolecular polar bonds to the benefit of the agent "parasiting" the surface. The analysis of the thermal factors first agree with a stabilization effect at low or moderate concentration of denaturants rapidly followed by a destabilization at specific weak points when the number of sites increase (overflooding effect).
About this Structure
2F4Y is a Single protein structure of sequence from Bacillus amyloliquefaciens with as ligand. Full crystallographic information is available from OCA.
Reference
On the edge of the denaturation process: application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations., Salem M, Mauguen Y, Prange T, Biochim Biophys Acta. 2006 May;1764(5):903-12. Epub 2006 Mar 20. PMID:16600702
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