2f8w
From Proteopedia
(New page: 200px<br /><applet load="2f8w" size="350" color="white" frame="true" align="right" spinBox="true" caption="2f8w, resolution 1.2Å" /> '''Crystal structure of ...) |
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==Overview== | ==Overview== | ||
| - | The crystal structure of a hexamer duplex d(CACGTG)(2) has been determined | + | The crystal structure of a hexamer duplex d(CACGTG)(2) has been determined and refined to an R-factor of 18.3% using X-ray data up to 1.2 A resolution. The sequence crystallizes as a left-handed Z-form double helix with Watson-Crick base pairing. There is one hexamer duplex, a spermine molecule, 71 water molecules, and an unexpected diamine (Z-5, 1,3-propanediamine, C(3)H(10)N(2)) in the asymmetric unit. This is the high-resolution non-disordered structure of a Z-DNA hexamer containing two AT base pairs in the interior of a duplex with no modifications such as bromination or methylation on cytosine bases. This structure does not possess multivalent cations such as cobalt hexaammine that are known to stabilize Z-DNA. The overall duplex structure and its crystal interactions are similar to those of the pure-spermine form of the d(CGCGCG)(2) structure. The spine of hydration in the minor groove is intact except in the vicinity of the T5A8 base pair. The binding of the Z-5 molecule in the minor grove of the d(CACGTG)(2) duplex appears to have a profound effect in conferring stability to a Z-DNA conformation via electrostatic complementarity and hydrogen bonding interactions. The successive base stacking geometry in d(CACGTG)(2) is similar to the corresponding steps in d(CG)(3). These results suggest that specific polyamines such as Z-5 could serve as powerful inducers of Z-type conformation in unmodified DNA sequences with AT base pairs. This structure provides a molecular basis for stabilizing AT base pairs incorporated into an alternating d(CG) sequence. |
==About this Structure== | ==About this Structure== | ||
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Interaction between the Z-type DNA duplex and 1,3-propanediamine: crystal structure of d(CACGTG)2 at 1.2 A resolution., Narayana N, Shamala N, Ganesh KN, Viswamitra MA, Biochemistry. 2006 Jan 31;45(4):1200-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16430216 16430216] | Interaction between the Z-type DNA duplex and 1,3-propanediamine: crystal structure of d(CACGTG)2 at 1.2 A resolution., Narayana N, Shamala N, Ganesh KN, Viswamitra MA, Biochemistry. 2006 Jan 31;45(4):1200-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16430216 16430216] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Ganesh, K | + | [[Category: Ganesh, K N.]] |
[[Category: Narayana, N.]] | [[Category: Narayana, N.]] | ||
[[Category: Shamala, N.]] | [[Category: Shamala, N.]] | ||
| - | [[Category: Viswamitra, M | + | [[Category: Viswamitra, M A.]] |
[[Category: 13D]] | [[Category: 13D]] | ||
[[Category: SPM]] | [[Category: SPM]] | ||
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[[Category: z-dna]] | [[Category: z-dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:57 2008'' |
Revision as of 15:18, 21 February 2008
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Crystal structure of d(CACGTG)2
Overview
The crystal structure of a hexamer duplex d(CACGTG)(2) has been determined and refined to an R-factor of 18.3% using X-ray data up to 1.2 A resolution. The sequence crystallizes as a left-handed Z-form double helix with Watson-Crick base pairing. There is one hexamer duplex, a spermine molecule, 71 water molecules, and an unexpected diamine (Z-5, 1,3-propanediamine, C(3)H(10)N(2)) in the asymmetric unit. This is the high-resolution non-disordered structure of a Z-DNA hexamer containing two AT base pairs in the interior of a duplex with no modifications such as bromination or methylation on cytosine bases. This structure does not possess multivalent cations such as cobalt hexaammine that are known to stabilize Z-DNA. The overall duplex structure and its crystal interactions are similar to those of the pure-spermine form of the d(CGCGCG)(2) structure. The spine of hydration in the minor groove is intact except in the vicinity of the T5A8 base pair. The binding of the Z-5 molecule in the minor grove of the d(CACGTG)(2) duplex appears to have a profound effect in conferring stability to a Z-DNA conformation via electrostatic complementarity and hydrogen bonding interactions. The successive base stacking geometry in d(CACGTG)(2) is similar to the corresponding steps in d(CG)(3). These results suggest that specific polyamines such as Z-5 could serve as powerful inducers of Z-type conformation in unmodified DNA sequences with AT base pairs. This structure provides a molecular basis for stabilizing AT base pairs incorporated into an alternating d(CG) sequence.
About this Structure
2F8W is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Interaction between the Z-type DNA duplex and 1,3-propanediamine: crystal structure of d(CACGTG)2 at 1.2 A resolution., Narayana N, Shamala N, Ganesh KN, Viswamitra MA, Biochemistry. 2006 Jan 31;45(4):1200-11. PMID:16430216
Page seeded by OCA on Thu Feb 21 17:18:57 2008
Categories: Protein complex | Ganesh, K N. | Narayana, N. | Shamala, N. | Viswamitra, M A. | 13D | SPM | 1 | 3-propanediamine | D(cacgtg) | Polyamine | Z-dna
