2f9g
From Proteopedia
(New page: 200px<br /><applet load="2f9g" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f9g, resolution 2.1Å" /> '''Crystal structure of ...) |
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| - | [[Image:2f9g.gif|left|200px]]<br /><applet load="2f9g" size=" | + | [[Image:2f9g.gif|left|200px]]<br /><applet load="2f9g" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2f9g, resolution 2.1Å" /> | caption="2f9g, resolution 2.1Å" /> | ||
'''Crystal structure of Fus3 phosphorylated on Tyr182'''<br /> | '''Crystal structure of Fus3 phosphorylated on Tyr182'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Scaffold proteins organize signaling proteins into pathways and are often | + | Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties. |
==About this Structure== | ==About this Structure== | ||
| - | 2F9G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http:// | + | 2F9G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F9G OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bashor, C | + | [[Category: Bashor, C J.]] |
| - | [[Category: Bhattacharyya, R | + | [[Category: Bhattacharyya, R P.]] |
| - | [[Category: Falick, A | + | [[Category: Falick, A M.]] |
| - | [[Category: Good, M | + | [[Category: Good, M C.]] |
| - | [[Category: Lim, W | + | [[Category: Lim, W A.]] |
[[Category: Remenyi, A.]] | [[Category: Remenyi, A.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
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[[Category: map kinase]] | [[Category: map kinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:19:05 2008'' |
Revision as of 15:19, 21 February 2008
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Crystal structure of Fus3 phosphorylated on Tyr182
Overview
Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.
About this Structure
2F9G is a Single protein structure of sequence from Saccharomyces cerevisiae with and as ligands. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
Reference
The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway., Bhattacharyya RP, Remenyi A, Good MC, Bashor CJ, Falick AM, Lim WA, Science. 2006 Feb 10;311(5762):822-6. Epub 2006 Jan 19. PMID:16424299
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