2fbd
From Proteopedia
(New page: 200px<br /><applet load="2fbd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fbd, resolution 1.90Å" /> '''The crystallographic...) |
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| - | [[Image:2fbd.jpg|left|200px]]<br /><applet load="2fbd" size=" | + | [[Image:2fbd.jpg|left|200px]]<br /><applet load="2fbd" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2fbd, resolution 1.90Å" /> | caption="2fbd, resolution 1.90Å" /> | ||
'''The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.'''<br /> | '''The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Lysozymes are mostly known for their defensive role against bacteria, but | + | Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate or PEG/2-propanol as the precipitant. X-ray diffraction data were collected to a maximum resolution of 1.9 angstroms using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P2(1) (unit-cell parameters a = 36.52, b = 79.44, c = 45.20 angstroms, beta = 102.97 degrees) and the orthorhombic space group P2(1)2(1)2 (unit-cell parameters a = 73.90, b = 96.40, c = 33.27 angstroms), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress. |
==About this Structure== | ==About this Structure== | ||
| - | 2FBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Musca_domestica Musca domestica] with SO4 and PEG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 2FBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Musca_domestica Musca domestica] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBD OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica., Marana SR, Cancado FC, Valerio AA, Ferreira C, Terra WR, Barbosa JA, Acta | + | Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica., Marana SR, Cancado FC, Valerio AA, Ferreira C, Terra WR, Barbosa JA, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):750-2. Epub 2006 Jul 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16880547 16880547] |
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Musca domestica]] | [[Category: Musca domestica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Barbosa, J | + | [[Category: Barbosa, J A.R G.]] |
| - | [[Category: Cancado, F | + | [[Category: Cancado, F C.]] |
| - | [[Category: Marana, S | + | [[Category: Marana, S R.]] |
[[Category: PEG]] | [[Category: PEG]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: musca domestica]] | [[Category: musca domestica]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:19:39 2008'' |
Revision as of 15:19, 21 February 2008
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The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.
Overview
Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate or PEG/2-propanol as the precipitant. X-ray diffraction data were collected to a maximum resolution of 1.9 angstroms using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P2(1) (unit-cell parameters a = 36.52, b = 79.44, c = 45.20 angstroms, beta = 102.97 degrees) and the orthorhombic space group P2(1)2(1)2 (unit-cell parameters a = 73.90, b = 96.40, c = 33.27 angstroms), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress.
About this Structure
2FBD is a Single protein structure of sequence from Musca domestica with and as ligands. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica., Marana SR, Cancado FC, Valerio AA, Ferreira C, Terra WR, Barbosa JA, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):750-2. Epub 2006 Jul 24. PMID:16880547
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