Sandbox Reserved 689
From Proteopedia
| Line 36: | Line 36: | ||
(Rifampicin crystal structure from Nakashima, et al. 2011) | (Rifampicin crystal structure from Nakashima, et al. 2011) | ||
| - | '''Minocycline''' is a lower molecular weight drug (~400 g/mol) that has been crystallized in the distal binding pocket. | + | '''Minocycline''' is a lower molecular weight drug (~400 g/mol) that has been crystallized in the <scene name='Sandbox_Reserved_689/Minocycline_acrb_binding/7'>distal binding pocket</scene>. |
| + | |||
| + | |||
== Energy Transduction == | == Energy Transduction == | ||
There are <scene name='Sandbox_Reserved_689/Proton_translocation_residues/1'>Four Titrateable Residues</scene> (Asp408, Asp407, Lys940, and Arg 971) whose protonation and deprotonation are suggested to play a large role in the conformational change between the L, T, and O states (Eicher, et al. 2009). PDB: 3D9B | There are <scene name='Sandbox_Reserved_689/Proton_translocation_residues/1'>Four Titrateable Residues</scene> (Asp408, Asp407, Lys940, and Arg 971) whose protonation and deprotonation are suggested to play a large role in the conformational change between the L, T, and O states (Eicher, et al. 2009). PDB: 3D9B | ||
Revision as of 14:41, 30 April 2013
| This Sandbox is Reserved from 30/01/2013, through 30/12/2013 for use in the course "Biochemistry II" taught by Hannah Tims at the Messiah College. This reservation includes Sandbox Reserved 686 through Sandbox Reserved 700. |
To get started:
More help: Help:Editing |
Contents |
The E. coli AcrB Efflux Pump
Structure
The AcrB efflux pump is part of a tripartite system used by E. Coli to remove antibiotic and other toxic molecules from the bacterial cell.
|
Structural Elements
Substrates
Because of the large binding pockets in AcrB, it is able to bind and export a wide variety of antibiotic drugs and other toxins.
Rifampicin is a high molecular weight drug (~800 g/mol) that has been crystallized in the proximal binding pocket
, interacting residues (all residues within 4 Angstroms) highlighted and shown in ball-and-stick representation.
interacting residues are shown here (Polar in blue, nonpolar in orange).
(switch loop is shown in orange).
(Rifampicin crystal structure from Nakashima, et al. 2011)
Minocycline is a lower molecular weight drug (~400 g/mol) that has been crystallized in the .
Energy Transduction
There are (Asp408, Asp407, Lys940, and Arg 971) whose protonation and deprotonation are suggested to play a large role in the conformational change between the L, T, and O states (Eicher, et al. 2009). PDB: 3D9B
