1rco

From Proteopedia

Revision as of 13:58, 30 October 2007

SPINACH RUBISCO IN COMPLEX WITH THE INHIBITOR D-XYLULOSE-2,2-DIOL-1,5-BISPHOSPHATE

Overview

Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyzes the, carboxylation of ribulose 1,5-bisphosphate. The reaction catalyzed by, Rubisco involves several steps, some of which can occur as partial, reactions, forming intermediates that can be isolated. Analogues of these, intermediates are potent inhibitors of the enzyme. We have studied the, interactions with the enzyme of two inhibitors, xylulose 1,5-bisphosphate, and 4-carboxyarabinitol 1,5-bisphosphate, by x-ray crystallography., Crystals of the complexes were formed by cocrystallization under, activating conditions. In addition, 4-carboxyarabinitol 1,5-bisphosphate, was soaked into preformed activated crystals of the enzyme. The result of, these experiments was the release of the activating CO2 molecule as well, as the ... [(full description)]

About this Structure

1RCO is a [Protein complex] structure of sequences from [Spinacia oleracea] with XDP as [ligand]. Active as [Ribulose-bisphosphate carboxylase], with EC number [4.1.1.39]. Structure known Active Sites: ACB, ACE, ACH, ACK, ACL, ACO, ACR and ACV. Full crystallographic information is available from [OCA].

Reference

A common structural basis for the inhibition of ribulose 1,5-bisphosphate carboxylase by 4-carboxyarabinitol 1,5-bisphosphate and xylulose 1,5-bisphosphate., Taylor TC, Fothergill MD, Andersson I, J Biol Chem. 1996 Dec 20;271(51):32894-9. PMID:8955130

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