1sfv

From Proteopedia

Revision as of 13:59, 30 October 2007

PORCINE PANCREAS PHOSPHOLIPASE A2, NMR, MINIMIZED AVERAGE STRUCTURE

Overview

The three-dimensional structure of porcine pancreatic PLA2 (PLA2), present, in a 40 kDa ternary complex with micelles and a competitive inhibitor, has, been determined using multidimensional heteronuclear NMR spectroscopy. The, structure of the protein (124 residues) is based on 1854 constraints, comprising 1792 distance and 62 phi torsion angle constraints. A total of, 18 structures was calculated using a combined approach of distance, geometry and restrained molecular dynamics. The atomic rms distribution, about the mean coordinate positions for residues 1-62 and 72-124 is 0.75, +/- 0.09 A for the backbone atoms and 1.14 +/- 0.10 A for all atoms. The, rms difference between the averaged minimized NMR structures of the free, PLA2 and PLA2 in the ternary complex is 3.5 A for the backbone ... [(full description)]

About this Structure

1SFV is a [Single protein] structure of sequence from [Sus scrofa] with CA as [ligand]. Active as [Phospholipase A(2)], with EC number [3.1.1.4]. Structure known Active Site: CA. Full crystallographic information is available from [OCA].

Reference

Solution structure of porcine pancreatic phospholipase A2 complexed with micelles and a competitive inhibitor., van den Berg B, Tessari M, Boelens R, Dijkman R, Kaptein R, de Haas GH, Verheij HM, J Biomol NMR. 1995 Feb;5(2):110-21. PMID:7703697

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