2fok
From Proteopedia
(New page: 200px<br /><applet load="2fok" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fok, resolution 2.30Å" /> '''STRUCTURE OF RESTRIC...) |
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| - | [[Image:2fok.jpg|left|200px]]<br /><applet load="2fok" size=" | + | [[Image:2fok.jpg|left|200px]]<br /><applet load="2fok" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2fok, resolution 2.30Å" /> | caption="2fok, resolution 2.30Å" /> | ||
'''STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI'''<br /> | '''STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | FokI is a member an unusual class of restriction enzymes that recognize a | + | FokI is a member an unusual class of restriction enzymes that recognize a specific DNA sequence and cleave nonspecifically a short distance away from that sequence. FokI consists of an N-terminal DNA recognition domain and a C-terminal cleavage domain. The bipartite nature of FokI has led to the development of artificial enzymes with novel specificities. We have solved the structure of FokI to 2.3 A resolution. The structure reveals a dimer, in which the dimerization interface is mediated by the cleavage domain. Each monomer has an overall conformation similar to that found in the FokI-DNA complex, with the cleavage domain packing alongside the DNA recognition domain. In corroboration with the cleavage data presented in the accompanying paper in this issue of Proceedings, we propose a model for FokI DNA cleavage that requires the dimerization of FokI on DNA to cleave both DNA strands. |
==About this Structure== | ==About this Structure== | ||
| - | 2FOK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Planomicrobium_okeanokoites Planomicrobium okeanokoites]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http:// | + | 2FOK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Planomicrobium_okeanokoites Planomicrobium okeanokoites]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FOK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Type II site-specific deoxyribonuclease]] | [[Category: Type II site-specific deoxyribonuclease]] | ||
| - | [[Category: Aggarwal, A | + | [[Category: Aggarwal, A K.]] |
[[Category: Bitinaite, J.]] | [[Category: Bitinaite, J.]] | ||
[[Category: Schildkraut, I.]] | [[Category: Schildkraut, I.]] | ||
| - | [[Category: Wah, D | + | [[Category: Wah, D A.]] |
[[Category: deoxyribonuclease]] | [[Category: deoxyribonuclease]] | ||
[[Category: dna cleavage]] | [[Category: dna cleavage]] | ||
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[[Category: type iis restriction endonuclease]] | [[Category: type iis restriction endonuclease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:23:32 2008'' |
Revision as of 15:23, 21 February 2008
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STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI
Overview
FokI is a member an unusual class of restriction enzymes that recognize a specific DNA sequence and cleave nonspecifically a short distance away from that sequence. FokI consists of an N-terminal DNA recognition domain and a C-terminal cleavage domain. The bipartite nature of FokI has led to the development of artificial enzymes with novel specificities. We have solved the structure of FokI to 2.3 A resolution. The structure reveals a dimer, in which the dimerization interface is mediated by the cleavage domain. Each monomer has an overall conformation similar to that found in the FokI-DNA complex, with the cleavage domain packing alongside the DNA recognition domain. In corroboration with the cleavage data presented in the accompanying paper in this issue of Proceedings, we propose a model for FokI DNA cleavage that requires the dimerization of FokI on DNA to cleave both DNA strands.
About this Structure
2FOK is a Single protein structure of sequence from Planomicrobium okeanokoites. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Full crystallographic information is available from OCA.
Reference
Structure of FokI has implications for DNA cleavage., Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK, Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. PMID:9724743
Page seeded by OCA on Thu Feb 21 17:23:32 2008
Categories: Planomicrobium okeanokoites | Single protein | Type II site-specific deoxyribonuclease | Aggarwal, A K. | Bitinaite, J. | Schildkraut, I. | Wah, D A. | Deoxyribonuclease | Dna cleavage | Dna hydrolysis | Dna-binding protein | Metal ion catalysis | Metalloenzyme | Nucleic acid recognition | Type iis restriction endonuclease
