2foy
From Proteopedia
(New page: 200px<br /> <applet load="2foy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2foy, resolution 1.55Å" /> '''Human Carbonic Anhy...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2foy.gif|left|200px]]<br /> | + | [[Image:2foy.gif|left|200px]]<br /><applet load="2foy" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2foy" size=" | + | |
caption="2foy, resolution 1.55Å" /> | caption="2foy, resolution 1.55Å" /> | ||
'''Human Carbonic Anhydrase I complexed with a two-prong inhibitor'''<br /> | '''Human Carbonic Anhydrase I complexed with a two-prong inhibitor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The atomic-resolution crystal structures of human carbonic anhydrases I | + | The atomic-resolution crystal structures of human carbonic anhydrases I and II complexed with "two-prong" inhibitors are reported. Each inhibitor contains a benzenesulfonamide prong and a cupric iminodiacetate (IDA-Cu(2+)) prong separated by linkers of different lengths and compositions. The ionized NH(-) group of each benzenesulfonamide coordinates to the active site Zn(2+) ion; the IDA-Cu(2+) prong of the tightest-binding inhibitor, BR30, binds to H64 of CAII and H200 of CAI. This work provides the first evidence verifying the structural basis of nanomolar affinity measured for two-prong inhibitors targeting the carbonic anhydrases. |
==About this Structure== | ==About this Structure== | ||
| - | 2FOY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN and B30 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http:// | + | 2FOY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=B30:'>B30</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FOY OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 14: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Banerjee, A | + | [[Category: Banerjee, A L.]] |
| - | [[Category: Christianson, D | + | [[Category: Christianson, D W.]] |
| - | [[Category: Haldar, M | + | [[Category: Haldar, M K.]] |
| - | [[Category: Jude, K | + | [[Category: Jude, K M.]] |
[[Category: Mallik, S.]] | [[Category: Mallik, S.]] | ||
[[Category: Manokaran, S.]] | [[Category: Manokaran, S.]] | ||
[[Category: Roy, B.]] | [[Category: Roy, B.]] | ||
| - | [[Category: Srivastava, D | + | [[Category: Srivastava, D K.]] |
[[Category: B30]] | [[Category: B30]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| Line 30: | Line 29: | ||
[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:23:41 2008'' |
Revision as of 15:23, 21 February 2008
|
Human Carbonic Anhydrase I complexed with a two-prong inhibitor
Overview
The atomic-resolution crystal structures of human carbonic anhydrases I and II complexed with "two-prong" inhibitors are reported. Each inhibitor contains a benzenesulfonamide prong and a cupric iminodiacetate (IDA-Cu(2+)) prong separated by linkers of different lengths and compositions. The ionized NH(-) group of each benzenesulfonamide coordinates to the active site Zn(2+) ion; the IDA-Cu(2+) prong of the tightest-binding inhibitor, BR30, binds to H64 of CAII and H200 of CAI. This work provides the first evidence verifying the structural basis of nanomolar affinity measured for two-prong inhibitors targeting the carbonic anhydrases.
About this Structure
2FOY is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Carbonate dehydratase, with EC number 4.2.1.1 Full crystallographic information is available from OCA.
Reference
Ultrahigh resolution crystal structures of human carbonic anhydrases I and II complexed with "two-prong" inhibitors reveal the molecular basis of high affinity., Jude KM, Banerjee AL, Haldar MK, Manokaran S, Roy B, Mallik S, Srivastava DK, Christianson DW, J Am Chem Soc. 2006 Mar 8;128(9):3011-8. PMID:16506782
Page seeded by OCA on Thu Feb 21 17:23:41 2008
Categories: Carbonate dehydratase | Homo sapiens | Single protein | Banerjee, A L. | Christianson, D W. | Haldar, M K. | Jude, K M. | Mallik, S. | Manokaran, S. | Roy, B. | Srivastava, D K. | B30 | ZN | Copper | Inhibitor | Lyase | Zinc
