2fq3
From Proteopedia
(New page: 200px<br /><applet load="2fq3" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fq3, resolution 1.4Å" /> '''Structure and functio...) |
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| - | [[Image:2fq3.gif|left|200px]]<br /><applet load="2fq3" size=" | + | [[Image:2fq3.gif|left|200px]]<br /><applet load="2fq3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2fq3, resolution 1.4Å" /> | caption="2fq3, resolution 1.4Å" /> | ||
'''Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes'''<br /> | '''Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The SWIRM domain is a module found in the Swi3 and Rsc8 subunits of | + | The SWIRM domain is a module found in the Swi3 and Rsc8 subunits of SWI/SNF-family chromatin remodeling complexes, and the Ada2 and BHC110/LSD1 subunits of chromatin modification complexes. Here we report the high-resolution crystal structure of the SWIRM domain from Swi3 and characterize the in vitro and in vivo function of the SWIRM domains from Saccharomyces cerevisiae Swi3 and Rsc8. The Swi3 SWIRM forms a four-helix bundle containing a pseudo 2-fold axis and a helix-turn-helix motif commonly found in DNA-binding proteins. We show that the Swi3 SWIRM binds free DNA and mononucleosomes with high and comparable affinity and that a subset of Swi3 substitution mutants that display growth defects in vivo also show impaired DNA-binding activity in vitro, consistent with a nucleosome targeting function of this domain. Genetic and biochemical studies also reveal that the Rsc8 and Swi3 SWIRM domains are essential for the proper assembly and in vivo functions of their respective complexes. Together, these studies identify the SWIRM domain as an essential multifunctional module for the regulation of gene expression. |
==About this Structure== | ==About this Structure== | ||
| - | 2FQ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 2FQ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQ3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cairns, B | + | [[Category: Cairns, B R.]] |
[[Category: Da, G.]] | [[Category: Da, G.]] | ||
[[Category: Lenkart, J.]] | [[Category: Lenkart, J.]] | ||
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[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:24:00 2008'' |
Revision as of 15:24, 21 February 2008
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Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes
Overview
The SWIRM domain is a module found in the Swi3 and Rsc8 subunits of SWI/SNF-family chromatin remodeling complexes, and the Ada2 and BHC110/LSD1 subunits of chromatin modification complexes. Here we report the high-resolution crystal structure of the SWIRM domain from Swi3 and characterize the in vitro and in vivo function of the SWIRM domains from Saccharomyces cerevisiae Swi3 and Rsc8. The Swi3 SWIRM forms a four-helix bundle containing a pseudo 2-fold axis and a helix-turn-helix motif commonly found in DNA-binding proteins. We show that the Swi3 SWIRM binds free DNA and mononucleosomes with high and comparable affinity and that a subset of Swi3 substitution mutants that display growth defects in vivo also show impaired DNA-binding activity in vitro, consistent with a nucleosome targeting function of this domain. Genetic and biochemical studies also reveal that the Rsc8 and Swi3 SWIRM domains are essential for the proper assembly and in vivo functions of their respective complexes. Together, these studies identify the SWIRM domain as an essential multifunctional module for the regulation of gene expression.
About this Structure
2FQ3 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes., Da G, Lenkart J, Zhao K, Shiekhattar R, Cairns BR, Marmorstein R, Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2057-62. Epub 2006 Feb 3. PMID:16461455
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