2fv4

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(New page: 200px<br /><applet load="2fv4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fv4" /> '''NMR solution structure of the yeast kinetoch...)
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[[Image:2fv4.gif|left|200px]]<br /><applet load="2fv4" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:2fv4.gif|left|200px]]<br /><applet load="2fv4" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2fv4" />
caption="2fv4" />
'''NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain'''<br />
'''NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain'''<br />
==Overview==
==Overview==
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The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during, mitosis. It is an approximately 570 A long, rod-shaped assembly of four, proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions, at either end of a central shaft. The complex bridges from the, centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular, end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2, globular end. We report the atomic structures of the Spc24/Spc25 globular, domain, determined both by X-ray crystallography at 1.9 A resolution and, by NMR. Spc24 and Spc25 fold tightly together into a single globular, entity with pseudo-2-fold symmetry. Conserved residues line a common, hydrophobic core and the bottom of a cleft, indicating that the functional, orthologs from other eukaryotes will have the same structure and, suggesting a docking site for components of the inner kinetochore.
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The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an approximately 570 A long, rod-shaped assembly of four proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore.
==About this Structure==
==About this Structure==
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2FV4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA].
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2FV4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FV4 OCA].
==Reference==
==Reference==
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Chou, J.J.]]
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[[Category: Chou, J J.]]
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[[Category: Schnell, J.R.]]
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[[Category: Schnell, J R.]]
[[Category: alpha-beta]]
[[Category: alpha-beta]]
[[Category: coiled-coil]]
[[Category: coiled-coil]]
[[Category: complex]]
[[Category: complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:47:16 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:23 2008''

Revision as of 15:25, 21 February 2008


2fv4

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NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain

Overview

The Ndc80 complex, a kinetochore component conserved from yeast to humans, is essential for proper chromosome alignment and segregation during mitosis. It is an approximately 570 A long, rod-shaped assembly of four proteins--Ndc80p (Hec1), Nuf2p, Spc24p, and Spc25p--with globular regions at either end of a central shaft. The complex bridges from the centromere-proximal inner kinetochore layer at its Spc24/Spc25 globular end to the microtubule binding outer kinetochore layer at its Ndc80/Nuf2 globular end. We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR. Spc24 and Spc25 fold tightly together into a single globular entity with pseudo-2-fold symmetry. Conserved residues line a common hydrophobic core and the bottom of a cleft, indicating that the functional orthologs from other eukaryotes will have the same structure and suggesting a docking site for components of the inner kinetochore.

About this Structure

2FV4 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain., Wei RR, Schnell JR, Larsen NA, Sorger PK, Chou JJ, Harrison SC, Structure. 2006 Jun;14(6):1003-9. PMID:16765893

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