2fwv
From Proteopedia
(New page: 200px<br /><applet load="2fwv" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fwv, resolution 1.700Å" /> '''Crystal Structure o...) |
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==Overview== | ==Overview== | ||
| - | The gene Rv0813c from Mycobacterium tuberculosis, which codes for a | + | The gene Rv0813c from Mycobacterium tuberculosis, which codes for a hypothetical protein of unknown function, is conserved within the order Actinomycetales but absent elsewhere. The crystal structure of Rv0813c reveals a new family of proteins that resemble the fatty acid-binding proteins (FABPs) found in eukaryotes. Rv0813c adopts the 10-stranded beta-barrel fold typical of FABPs but lacks the double-helix insert that covers the entry to the binding site in the eukaryotic proteins. The barrel encloses a deep cavity, at the bottom of which a small cyclic ligand was found to bind to the hydroxyl group of Tyr192. This residue is part of a conserved Arg-X-Tyr motif much like the triad that binds the carboxylate group of fatty acids in FABPs. Most of the residues forming the internal surface of the cavity are conserved in homologous protein sequences found in CG-rich prokaryotes, strongly suggesting that Rv0813c is a member of a new family of bacterial FABP-like proteins that may have roles in the recognition, transport, and/or storage of small molecules in the bacterial cytosol. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Alzari, P | + | [[Category: Alzari, P M.]] |
| - | [[Category: Betton, J | + | [[Category: Betton, J M.]] |
[[Category: Buschiazzo, A.]] | [[Category: Buschiazzo, A.]] | ||
| - | [[Category: Cole, S | + | [[Category: Cole, S T.]] |
[[Category: Grana, M.]] | [[Category: Grana, M.]] | ||
[[Category: Haouz, A.]] | [[Category: Haouz, A.]] | ||
| - | [[Category: SPINE, Structural | + | [[Category: SPINE, Structural Proteomics in Europe.]] |
[[Category: Shepard, W.]] | [[Category: Shepard, W.]] | ||
[[Category: DIO]] | [[Category: DIO]] | ||
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[[Category: unknown function]] | [[Category: unknown function]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:56 2008'' |
Revision as of 15:25, 21 February 2008
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Crystal Structure of Rv0813
Overview
The gene Rv0813c from Mycobacterium tuberculosis, which codes for a hypothetical protein of unknown function, is conserved within the order Actinomycetales but absent elsewhere. The crystal structure of Rv0813c reveals a new family of proteins that resemble the fatty acid-binding proteins (FABPs) found in eukaryotes. Rv0813c adopts the 10-stranded beta-barrel fold typical of FABPs but lacks the double-helix insert that covers the entry to the binding site in the eukaryotic proteins. The barrel encloses a deep cavity, at the bottom of which a small cyclic ligand was found to bind to the hydroxyl group of Tyr192. This residue is part of a conserved Arg-X-Tyr motif much like the triad that binds the carboxylate group of fatty acids in FABPs. Most of the residues forming the internal surface of the cavity are conserved in homologous protein sequences found in CG-rich prokaryotes, strongly suggesting that Rv0813c is a member of a new family of bacterial FABP-like proteins that may have roles in the recognition, transport, and/or storage of small molecules in the bacterial cytosol.
About this Structure
2FWV is a Single protein structure of sequence from Mycobacterium tuberculosis with and as ligands. Full crystallographic information is available from OCA.
Reference
The crystal structure of Rv0813c from Mycobacterium tuberculosis reveals a new family of fatty acid-binding protein-like proteins in bacteria., Shepard W, Haouz A, Grana M, Buschiazzo A, Betton JM, Cole ST, Alzari PM, J Bacteriol. 2007 Mar;189(5):1899-904. Epub 2006 Dec 15. PMID:17172346
Page seeded by OCA on Thu Feb 21 17:25:56 2008
Categories: Mycobacterium tuberculosis | Single protein | Alzari, P M. | Betton, J M. | Buschiazzo, A. | Cole, S T. | Grana, M. | Haouz, A. | SPINE, Structural Proteomics in Europe. | Shepard, W. | DIO | GOL | Conserved hypothetical | Fatty acid binding protein like | Restricted to actinomycetes | Spine | Structural genomics | Structural proteomics in europe | Unknown function
