2fx3

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(New page: 200px<br /><applet load="2fx3" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fx3, resolution 3.400&Aring;" /> '''Crystal Structure D...)
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[[Image:2fx3.gif|left|200px]]<br /><applet load="2fx3" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2fx3, resolution 3.400&Aring;" />
caption="2fx3, resolution 3.400&Aring;" />
'''Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set'''<br />
'''Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set'''<br />
==Overview==
==Overview==
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Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in, the presence of novel inhibitors. The only crystals which could be grown, were epitaxially as well as merohedrally twinned, highly mosaic and, diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell, parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120, degrees . To determine whether an inhibitor was present in the crystal, a, poor-quality X-ray diffraction data set had to be processed. The, three-dimensional structure was ultimately solved and the original, question answered. The results also reveal a new type of dimer packing for, EF-Tu-GDP.
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Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in the presence of novel inhibitors. The only crystals which could be grown were epitaxially as well as merohedrally twinned, highly mosaic and diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120 degrees . To determine whether an inhibitor was present in the crystal, a poor-quality X-ray diffraction data set had to be processed. The three-dimensional structure was ultimately solved and the original question answered. The results also reveal a new type of dimer packing for EF-Tu-GDP.
==About this Structure==
==About this Structure==
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2FX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FX3 OCA].
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2FX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FX3 OCA].
==Reference==
==Reference==
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Heffron, S.E.]]
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[[Category: Heffron, S E.]]
[[Category: Jurnak, F.]]
[[Category: Jurnak, F.]]
[[Category: Moeller, R.]]
[[Category: Moeller, R.]]
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[[Category: merohedral twinning]]
[[Category: merohedral twinning]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:49:13 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:56 2008''

Revision as of 15:25, 21 February 2008

Image:2fx3.gif

2fx3, resolution 3.400Å

Drag the structure with the mouse to rotate

Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set

Overview

Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in the presence of novel inhibitors. The only crystals which could be grown were epitaxially as well as merohedrally twinned, highly mosaic and diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120 degrees . To determine whether an inhibitor was present in the crystal, a poor-quality X-ray diffraction data set had to be processed. The three-dimensional structure was ultimately solved and the original question answered. The results also reveal a new type of dimer packing for EF-Tu-GDP.

About this Structure

2FX3 is a Single protein structure of sequence from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.

Reference

Solving the structure of Escherichia coli elongation factor Tu using a twinned data set., Heffron SE, Moeller R, Jurnak F, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):433-8. Epub 2006, Mar 18. PMID:16552145

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