2fz2
From Proteopedia
(New page: 200px<br /><applet load="2fz2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fz2, resolution 2.90Å" /> '''Structure of Turnip ...) |
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| - | [[Image:2fz2.gif|left|200px]]<br /><applet load="2fz2" size=" | + | [[Image:2fz2.gif|left|200px]]<br /><applet load="2fz2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2fz2, resolution 2.90Å" /> | caption="2fz2, resolution 2.90Å" /> | ||
'''Structure of Turnip Yellow Mosaic Virus at 100 K'''<br /> | '''Structure of Turnip Yellow Mosaic Virus at 100 K'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Difference electron density maps, based on structure factor amplitudes and | + | Difference electron density maps, based on structure factor amplitudes and experimental phases from crystals of wild-type turnip yellow mosaic virus and those of empty capsids prepared by freeze-thawing, show a large portion of the encapsidated RNA to have an icosahedral distribution. Four unique segments of base-paired, double-helical RNA, one to two turns in length, lie between 33-A and 101-A radius and are organized about either 2-fold or 5-fold icosahedral axes. In addition, single-stranded loops of RNA invade the pentameric and hexameric capsomeres where they contact the interior capsid surface. The remaining RNA, not seen in electron density maps, must serve as connecting links between these secondary structural elements and is likely icosahedrally disordered. The distribution of RNA observed crystallographically appears to be in agreement with models based on biochemical data and secondary structural analyses. |
==About this Structure== | ==About this Structure== | ||
| - | 2FZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Turnip_yellow_mosaic_virus_(isolate_australia) Turnip yellow mosaic virus (isolate australia)]. Full crystallographic information is available from [http:// | + | 2FZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Turnip_yellow_mosaic_virus_(isolate_australia) Turnip yellow mosaic virus (isolate australia)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZ2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Turnip yellow mosaic virus (isolate australia)]] | [[Category: Turnip yellow mosaic virus (isolate australia)]] | ||
| - | [[Category: Larson, S | + | [[Category: Larson, S B.]] |
| - | [[Category: Lucas, R | + | [[Category: Lucas, R W.]] |
[[Category: McPherson, A.]] | [[Category: McPherson, A.]] | ||
[[Category: capsid protein]] | [[Category: capsid protein]] | ||
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[[Category: tymv]] | [[Category: tymv]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:26:35 2008'' |
Revision as of 15:26, 21 February 2008
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Structure of Turnip Yellow Mosaic Virus at 100 K
Overview
Difference electron density maps, based on structure factor amplitudes and experimental phases from crystals of wild-type turnip yellow mosaic virus and those of empty capsids prepared by freeze-thawing, show a large portion of the encapsidated RNA to have an icosahedral distribution. Four unique segments of base-paired, double-helical RNA, one to two turns in length, lie between 33-A and 101-A radius and are organized about either 2-fold or 5-fold icosahedral axes. In addition, single-stranded loops of RNA invade the pentameric and hexameric capsomeres where they contact the interior capsid surface. The remaining RNA, not seen in electron density maps, must serve as connecting links between these secondary structural elements and is likely icosahedrally disordered. The distribution of RNA observed crystallographically appears to be in agreement with models based on biochemical data and secondary structural analyses.
About this Structure
2FZ2 is a Single protein structure of sequence from Turnip yellow mosaic virus (isolate australia). Full crystallographic information is available from OCA.
Reference
The RNA of turnip yellow mosaic virus exhibits icosahedral order., Larson SB, Lucas RW, Greenwood A, McPherson A, Virology. 2005 Apr 10;334(2):245-54. PMID:15780874
Page seeded by OCA on Thu Feb 21 17:26:35 2008
