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2g23
From Proteopedia
(New page: 200px<br /><applet load="2g23" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g23, resolution 2.30Å" /> '''The crystal structur...) |
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| - | [[Image:2g23.gif|left|200px]]<br /><applet load="2g23" size=" | + | [[Image:2g23.gif|left|200px]]<br /><applet load="2g23" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2g23, resolution 2.30Å" /> | caption="2g23, resolution 2.30Å" /> | ||
'''The crystal structure of hexameric phenoxazinone synthase'''<br /> | '''The crystal structure of hexameric phenoxazinone synthase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the | + | The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 A and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 A with a large central cavity that has a diameter of 50 A. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure. |
==About this Structure== | ==About this Structure== | ||
| - | 2G23 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus] with CU, C2O and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2G23 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=C2O:'>C2O</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G23 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces antibioticus]] | [[Category: Streptomyces antibioticus]] | ||
| - | [[Category: Allen, J | + | [[Category: Allen, J P.]] |
[[Category: Camara-Artigas, A.]] | [[Category: Camara-Artigas, A.]] | ||
| - | [[Category: Francisco, W | + | [[Category: Francisco, W A.]] |
| - | [[Category: Smith, A | + | [[Category: Smith, A W.]] |
[[Category: Wang, M.]] | [[Category: Wang, M.]] | ||
[[Category: C2O]] | [[Category: C2O]] | ||
| Line 27: | Line 27: | ||
[[Category: multicopper oxidase]] | [[Category: multicopper oxidase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:27:26 2008'' |
Revision as of 15:27, 21 February 2008
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The crystal structure of hexameric phenoxazinone synthase
Overview
The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 A and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 A with a large central cavity that has a diameter of 50 A. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure.
About this Structure
2G23 is a Single protein structure of sequence from Streptomyces antibioticus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center., Smith AW, Camara-Artigas A, Wang M, Allen JP, Francisco WA, Biochemistry. 2006 Apr 11;45(14):4378-87. PMID:16584173
Page seeded by OCA on Thu Feb 21 17:27:26 2008
Categories: Single protein | Streptomyces antibioticus | Allen, J P. | Camara-Artigas, A. | Francisco, W A. | Smith, A W. | Wang, M. | C2O | CU | GOL | Copper | Hexamer | Laccase | Metalloprotein | Multicopper oxidase
