2g30
From Proteopedia
(New page: 200px<br /> <applet load="2g30" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g30, resolution 1.600Å" /> '''beta appendage of ...) |
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| - | [[Image:2g30.gif|left|200px]]<br /> | + | [[Image:2g30.gif|left|200px]]<br /><applet load="2g30" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2g30" size=" | + | |
caption="2g30, resolution 1.600Å" /> | caption="2g30, resolution 1.600Å" /> | ||
'''beta appendage of AP2 complexed with ARH peptide'''<br /> | '''beta appendage of AP2 complexed with ARH peptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Clathrin-associated sorting proteins (CLASPs) expand the repertoire of | + | Clathrin-associated sorting proteins (CLASPs) expand the repertoire of endocytic cargo sorted into clathrin-coated vesicles beyond the transmembrane proteins that bind physically to the AP-2 adaptor. LDL and GPCRs are internalized by ARH and beta-arrestin, respectively. We show that these two CLASPs bind selectively to the AP-2 beta2 appendage platform via an alpha-helical [DE](n)X(1-2)FXX[FL]XXXR motif, and that this motif also occurs and is functional in the epsins. In beta-arrestin, this motif maintains the endocytosis-incompetent state by binding back on the folded core of the protein in a beta strand conformation. Triggered via a beta-arrestin/GPCR interaction, the motif must be displaced and must undergo a strand to helix transition to enable the beta2 appendage binding that drives GPCR-beta-arrestin complexes into clathrin coats. Another interaction surface on the beta2 appendage sandwich is identified for proteins such as eps15 and clathrin, suggesting a mechanism by which clathrin displaces eps15 to lattice edges during assembly. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2G30 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 2G30 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb88_1.html Clathrin]]. Full crystallographic information is available from [http:// | + | 2G30 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 2G30 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb88_1.html Clathrin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G30 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Collins, B | + | [[Category: Collins, B M.]] |
| - | [[Category: Edeling, M | + | [[Category: Edeling, M A.]] |
| - | [[Category: Owen, D | + | [[Category: Owen, D J.]] |
| - | [[Category: Traub, L | + | [[Category: Traub, L M.]] |
[[Category: adaptor]] | [[Category: adaptor]] | ||
[[Category: alpha-helical arh peptide]] | [[Category: alpha-helical arh peptide]] | ||
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[[Category: sandwich domain]] | [[Category: sandwich domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:27:38 2008'' |
Revision as of 15:27, 21 February 2008
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beta appendage of AP2 complexed with ARH peptide
Contents |
Overview
Clathrin-associated sorting proteins (CLASPs) expand the repertoire of endocytic cargo sorted into clathrin-coated vesicles beyond the transmembrane proteins that bind physically to the AP-2 adaptor. LDL and GPCRs are internalized by ARH and beta-arrestin, respectively. We show that these two CLASPs bind selectively to the AP-2 beta2 appendage platform via an alpha-helical [DE](n)X(1-2)FXX[FL]XXXR motif, and that this motif also occurs and is functional in the epsins. In beta-arrestin, this motif maintains the endocytosis-incompetent state by binding back on the folded core of the protein in a beta strand conformation. Triggered via a beta-arrestin/GPCR interaction, the motif must be displaced and must undergo a strand to helix transition to enable the beta2 appendage binding that drives GPCR-beta-arrestin complexes into clathrin coats. Another interaction surface on the beta2 appendage sandwich is identified for proteins such as eps15 and clathrin, suggesting a mechanism by which clathrin displaces eps15 to lattice edges during assembly.
Disease
Known diseases associated with this structure: Hypercholesterolemia, familial, autosomal recessive OMIM:[605747]
About this Structure
2G30 is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 2G30 with [Clathrin]. Full crystallographic information is available from OCA.
Reference
Molecular switches involving the AP-2 beta2 appendage regulate endocytic cargo selection and clathrin coat assembly., Edeling MA, Mishra SK, Keyel PA, Steinhauser AL, Collins BM, Roth R, Heuser JE, Owen DJ, Traub LM, Dev Cell. 2006 Mar;10(3):329-42. PMID:16516836
Page seeded by OCA on Thu Feb 21 17:27:38 2008
