2g5b
From Proteopedia
(New page: 200px<br /> <applet load="2g5b" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g5b, resolution 2.300Å" /> '''Crystal Structure ...) |
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| - | [[Image:2g5b.gif|left|200px]]<br /> | + | [[Image:2g5b.gif|left|200px]]<br /><applet load="2g5b" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2g5b" size=" | + | |
caption="2g5b, resolution 2.300Å" /> | caption="2g5b, resolution 2.300Å" /> | ||
'''Crystal Structure of the anti-Bax monoclonal antibody 6A7 and a Bax peptide.'''<br /> | '''Crystal Structure of the anti-Bax monoclonal antibody 6A7 and a Bax peptide.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Bcl-2 family member Bax plays a critical role in apoptosis. In healthy | + | The Bcl-2 family member Bax plays a critical role in apoptosis. In healthy resting cells, Bax resides in the cytoplasm and loosely attached to the mitochondrial membrane. Apoptotic stimuli induce Bax activation, which is characterized by translocation and multimerization on the mitochondrial membrane surface resulting in exposure of an amino terminal epitope recognized by the monoclonal antibody 6A7. To understand the structural changes that occur during Bax activation, we determined the crystal structure of a Bax peptide bound to the 6A7 Fab fragment to a resolution of 2.3 A. The structure reveals the conformation of the 6A7 peptide epitope on Bax in the activated form and elucidates the extensive structural changes that Bax must undergo during the conversion from its native to its activated conformation. |
==About this Structure== | ==About this Structure== | ||
| - | 2G5B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2G5B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Dai, S.]] | [[Category: Dai, S.]] | ||
| - | [[Category: Kappler, J | + | [[Category: Kappler, J W.]] |
[[Category: Marrack, P.]] | [[Category: Marrack, P.]] | ||
| - | [[Category: Murphy, G | + | [[Category: Murphy, G A.]] |
| - | [[Category: Peyerl, F | + | [[Category: Peyerl, F W.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: 6a7]] | [[Category: 6a7]] | ||
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[[Category: fab-peptide complex]] | [[Category: fab-peptide complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:28:19 2008'' |
Revision as of 15:28, 21 February 2008
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Crystal Structure of the anti-Bax monoclonal antibody 6A7 and a Bax peptide.
Overview
The Bcl-2 family member Bax plays a critical role in apoptosis. In healthy resting cells, Bax resides in the cytoplasm and loosely attached to the mitochondrial membrane. Apoptotic stimuli induce Bax activation, which is characterized by translocation and multimerization on the mitochondrial membrane surface resulting in exposure of an amino terminal epitope recognized by the monoclonal antibody 6A7. To understand the structural changes that occur during Bax activation, we determined the crystal structure of a Bax peptide bound to the 6A7 Fab fragment to a resolution of 2.3 A. The structure reveals the conformation of the 6A7 peptide epitope on Bax in the activated form and elucidates the extensive structural changes that Bax must undergo during the conversion from its native to its activated conformation.
About this Structure
2G5B is a Protein complex structure of sequences from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Elucidation of some Bax conformational changes through crystallization of an antibody-peptide complex., Peyerl FW, Dai S, Murphy GA, Crawford F, White J, Marrack P, Kappler JW, Cell Death Differ. 2007 Mar;14(3):447-52. Epub 2006 Sep 1. PMID:16946732
Page seeded by OCA on Thu Feb 21 17:28:19 2008
Categories: Mus musculus | Protein complex | Dai, S. | Kappler, J W. | Marrack, P. | Murphy, G A. | Peyerl, F W. | SO4 | 6a7 | Antibody | Bax | Fab | Fab-peptide complex
