2g5q
From Proteopedia
(New page: 200px<br /><applet load="2g5q" size="350" color="white" frame="true" align="right" spinBox="true" caption="2g5q, resolution 2.700Å" /> '''Crystal structures ...) |
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==Overview== | ==Overview== | ||
| - | Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the | + | Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the 2-deoxystreptamine ring (ring II) by the L-haba group. In the present study, the crystal structure of a complex between oligonucleotide containing the bacterial ribosomal A site and amikacin has been solved at 2.7 A resolution. Amikacin specifically binds to the A site in practically the same way as its parent compound kanamycin. In addition, the L-haba group interacts with the upper side of the A site through two direct contacts, O2*...H-N4(C1496) and N4*-H...O6(G1497). The present crystal structure shows how the introduction of the L-haba group on ring II of aminoglycoside is an effective mutation for obtaining a higher affinity to the bacterial A site. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Francois, B.]] | [[Category: Francois, B.]] | ||
[[Category: Kondo, J.]] | [[Category: Kondo, J.]] | ||
| - | [[Category: Murray, J | + | [[Category: Murray, J B.]] |
| - | [[Category: Russell, R | + | [[Category: Russell, R J.M.]] |
[[Category: Westhof, E.]] | [[Category: Westhof, E.]] | ||
[[Category: AKN]] | [[Category: AKN]] | ||
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[[Category: x-ray analysis]] | [[Category: x-ray analysis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:28:27 2008'' |
Revision as of 15:28, 21 February 2008
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Crystal structures of the bacterial ribosomal decoding site complexed with amikacin.
Overview
Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the 2-deoxystreptamine ring (ring II) by the L-haba group. In the present study, the crystal structure of a complex between oligonucleotide containing the bacterial ribosomal A site and amikacin has been solved at 2.7 A resolution. Amikacin specifically binds to the A site in practically the same way as its parent compound kanamycin. In addition, the L-haba group interacts with the upper side of the A site through two direct contacts, O2*...H-N4(C1496) and N4*-H...O6(G1497). The present crystal structure shows how the introduction of the L-haba group on ring II of aminoglycoside is an effective mutation for obtaining a higher affinity to the bacterial A site.
About this Structure
2G5Q is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the bacterial ribosomal decoding site complexed with amikacin containing the gamma-amino-alpha-hydroxybutyryl (haba) group., Kondo J, Francois B, Russell RJ, Murray JB, Westhof E, Biochimie. 2006 Aug;88(8):1027-31. Epub 2006 Jun 13. PMID:16806634
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