2ga0
From Proteopedia
(New page: 200px<br /><applet load="2ga0" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ga0, resolution 2.700Å" /> '''Variable Small Prot...) |
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==Overview== | ==Overview== | ||
| - | Vsp surface lipoproteins are serotype-defining antigens of relapsing fever | + | Vsp surface lipoproteins are serotype-defining antigens of relapsing fever spirochetes that undergo multiphasic antigenic variation to allow bacterial persistence in spite of an immune response. Two isogenic serotypes of Borrelia turicatae strain Oz1 differ in their Vsp sequences and in disease manifestations in infected mice: Vsp1 is associated with the selection of a neurological niche, while Vsp2 is associated with blood and skin infection. We report here crystal structures of the Vsp1 dimer at 2.7 and 2.2 A. The structures confirm that relapsing fever Vsp proteins share a common helical fold with OspCs of Lyme disease-causing Borrelia. The fold features an inner stem formed by highly conserved N and C termini and an outer "dome" formed by the variable central residues. Both Vsp1 and OspC structures possess small water-filled cavities, or pockets, that are lined largely by variable residues and are thus highly variable in shape. These features appear to signify tolerance of the Vsp-OspC fold for imperfect packing of residues at its antigenic surface. Structural comparison of Vsp1 with a homology model for Vsp2 suggests that observed differences in disease manifestation may arise in part from distinct differences in electrostatic surface properties; additional predicted positively charged surface patches on Vsp2 compared to Vsp1 may be sufficient to explain the relative propensity of Vsp2 to bind to acidic glycosaminoglycans. |
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Borrelia turicatae]] | [[Category: Borrelia turicatae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Barbour, A | + | [[Category: Barbour, A G.]] |
| - | [[Category: Lawson, C | + | [[Category: Lawson, C L.]] |
| - | [[Category: Yung, B | + | [[Category: Yung, B H.]] |
| - | [[Category: Zuckert, W | + | [[Category: Zuckert, W R.]] |
[[Category: NI]] | [[Category: NI]] | ||
[[Category: helical bundle]] | [[Category: helical bundle]] | ||
[[Category: ni(ii) binding sites]] | [[Category: ni(ii) binding sites]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:29:42 2008'' |
Revision as of 15:29, 21 February 2008
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Variable Small Protein 1 of Borrelia turicatae (VspA or Vsp1)
Overview
Vsp surface lipoproteins are serotype-defining antigens of relapsing fever spirochetes that undergo multiphasic antigenic variation to allow bacterial persistence in spite of an immune response. Two isogenic serotypes of Borrelia turicatae strain Oz1 differ in their Vsp sequences and in disease manifestations in infected mice: Vsp1 is associated with the selection of a neurological niche, while Vsp2 is associated with blood and skin infection. We report here crystal structures of the Vsp1 dimer at 2.7 and 2.2 A. The structures confirm that relapsing fever Vsp proteins share a common helical fold with OspCs of Lyme disease-causing Borrelia. The fold features an inner stem formed by highly conserved N and C termini and an outer "dome" formed by the variable central residues. Both Vsp1 and OspC structures possess small water-filled cavities, or pockets, that are lined largely by variable residues and are thus highly variable in shape. These features appear to signify tolerance of the Vsp-OspC fold for imperfect packing of residues at its antigenic surface. Structural comparison of Vsp1 with a homology model for Vsp2 suggests that observed differences in disease manifestation may arise in part from distinct differences in electrostatic surface properties; additional predicted positively charged surface patches on Vsp2 compared to Vsp1 may be sufficient to explain the relative propensity of Vsp2 to bind to acidic glycosaminoglycans.
About this Structure
2GA0 is a Single protein structure of sequence from Borrelia turicatae with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of neurotropism-associated variable surface protein 1 (Vsp1) of Borrelia turicatae., Lawson CL, Yung BH, Barbour AG, Zuckert WR, J Bacteriol. 2006 Jun;188(12):4522-30. PMID:16740958
Page seeded by OCA on Thu Feb 21 17:29:42 2008
