2ga9
From Proteopedia
(New page: 200px<br /><applet load="2ga9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ga9, resolution 2.300Å" /> '''Crystal Structure o...) |
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| - | [[Image:2ga9.gif|left|200px]]<br /><applet load="2ga9" size=" | + | [[Image:2ga9.gif|left|200px]]<br /><applet load="2ga9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ga9, resolution 2.300Å" /> | caption="2ga9, resolution 2.300Å" /> | ||
'''Crystal Structure of the Heterodimeric Vaccinia Virus Polyadenylate Polymerase with Bound ATP-gamma-S'''<br /> | '''Crystal Structure of the Heterodimeric Vaccinia Virus Polyadenylate Polymerase with Bound ATP-gamma-S'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Polyadenylation of mRNAs in poxviruses, crucial for virion maturation, is | + | Polyadenylation of mRNAs in poxviruses, crucial for virion maturation, is carried out by a poly(A) polymerase heterodimer composed of a catalytic component, VP55, and a processivity factor, VP39. The ATP-gamma-S bound and unbound crystal structures of the vaccinia polymerase reveal an unusual architecture for VP55 that comprises of N-terminal, central or catalytic, and C-terminal domains with different topologies and that differs from many polymerases, including the eukaryotic poly(A) polymerases. Residues in the active site of VP55, located between the catalytic and C-terminal domains, make specific interactions with the adenine of the ATP analog, establishing the molecular basis of ATP recognition. VP55's concave surface docks the globular VP39. A model for RNA primer binding that involves all three VP55 domains and VP39 is proposed. The model supports biochemical evidence that VP39 functions as a processivity factor by partially enclosing the RNA primer at the heterodimer interface. |
==About this Structure== | ==About this Structure== | ||
| - | 2GA9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus] with CA and SAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/mRNA_(nucleoside-2'-O-)-methyltransferase mRNA (nucleoside-2'-O-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.57 2.1.1.57] Full crystallographic information is available from [http:// | + | 2GA9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=SAP:'>SAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/mRNA_(nucleoside-2'-O-)-methyltransferase mRNA (nucleoside-2'-O-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.57 2.1.1.57] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GA9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Vaccinia virus]] | [[Category: Vaccinia virus]] | ||
[[Category: mRNA (nucleoside-2'-O-)-methyltransferase]] | [[Category: mRNA (nucleoside-2'-O-)-methyltransferase]] | ||
| - | [[Category: Bowman, B | + | [[Category: Bowman, B R.]] |
| - | [[Category: Gershon, P | + | [[Category: Gershon, P D.]] |
| - | [[Category: Moure, C | + | [[Category: Moure, C M.]] |
| - | [[Category: Quiocho, F | + | [[Category: Quiocho, F A.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: SAP]] | [[Category: SAP]] | ||
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[[Category: processivity]] | [[Category: processivity]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:29:45 2008'' |
Revision as of 15:29, 21 February 2008
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Crystal Structure of the Heterodimeric Vaccinia Virus Polyadenylate Polymerase with Bound ATP-gamma-S
Overview
Polyadenylation of mRNAs in poxviruses, crucial for virion maturation, is carried out by a poly(A) polymerase heterodimer composed of a catalytic component, VP55, and a processivity factor, VP39. The ATP-gamma-S bound and unbound crystal structures of the vaccinia polymerase reveal an unusual architecture for VP55 that comprises of N-terminal, central or catalytic, and C-terminal domains with different topologies and that differs from many polymerases, including the eukaryotic poly(A) polymerases. Residues in the active site of VP55, located between the catalytic and C-terminal domains, make specific interactions with the adenine of the ATP analog, establishing the molecular basis of ATP recognition. VP55's concave surface docks the globular VP39. A model for RNA primer binding that involves all three VP55 domains and VP39 is proposed. The model supports biochemical evidence that VP39 functions as a processivity factor by partially enclosing the RNA primer at the heterodimer interface.
About this Structure
2GA9 is a Protein complex structure of sequences from Vaccinia virus with and as ligands. Active as mRNA (nucleoside-2'-O-)-methyltransferase, with EC number 2.1.1.57 Full crystallographic information is available from OCA.
Reference
Crystal structures of the vaccinia virus polyadenylate polymerase heterodimer: insights into ATP selectivity and processivity., Moure CM, Bowman BR, Gershon PD, Quiocho FA, Mol Cell. 2006 May 5;22(3):339-49. PMID:16678106
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